Effects of Acupuncture at Tianshu(ST25)and Shangjuxu(ST37)on the Intestinal Mucosa and Metabolites of Local Skin Tissues at Tianshu(ST25)in 2,4,6-Trinitrobenzene Sulfonic Acid and Ethanol-induced Rats  

作　　者：Jing-Ying Zhou Yi-Tian Lai Ling Ren Lei Lan Guo-Shan Zhang Mi Liu 

机构地区：[1]Department of Acupuncture and Moxibustion,College of Acupuncture,Moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha,Hunan,China [2]Department of Professional Acupuncture and Moxibustion,College of Acupuncture,Moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha,Hunan,China [3]Michael G.DeGroote Institute for Pain Research and Care,McMaster University,Hamilton,Ontario,Canada

出　　处：《World Journal of Traditional Chinese Medicine》2023年第2期131-140,共10页世界中医药杂志（英文）

基　　金：National Natural Science Foundation of China(No.81603705,No.81774438);China Postdoctoral Science Foundation Project(No.2107M612567);Natural Science Foundation of Hunan Province(No.2021JJ30513,No.2017JJ3245);Scientific Research Project of Hunan Provincial Department of Education(No.21A0235,No.20B444);Hunan Traditional Chinese Medicine Research Project(No.C2022027);Hunan Province Youth Backbone Teacher Training Target Project(2021);Changsha Natural Science Foundation(No.kq2208183).

摘　　要：Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal group.Four normal rats and four CD modeling rats were selected at random after model identification was established.Furthermore,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture group.Rats in the acupuncture group underwent acupuncture of Tianshu(ST25)and Shangjuxu(ST37)for 7 days.After the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)staining.The local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in rats.Results:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell infiltration.In the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly improved.The choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P<0.05 or P<0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P<0.05 or P<0.01).Valine,leucine,and L-isoleucine biosynthesis and

关 键 词：ACUPUNCTURE Crohn's disease METABONOMICS point Tianshu(ST25) 

分 类 号：R245[医药卫生—针灸推拿学]