Extraction,Purification,and Anti-Inflammatory Activity of Steroid Fraction from Physalis Alkekengi L.Var.Franchetii(Mast.)Makino  

作　　者：Tian-Yi Xia Yi Wang Yan-Ni Yang Wu-Jing Wang Zi-He Ding Ren-Xing Zhong Ying Chen Wei Li Ming-Ming Peng Chuan-Qiu Li Li-Feng Shang Bing Liu Zhen-Yue Wang Chong-Rong Shi Zun-Peng Shu 

机构地区：[1]Guangdong Standardized Processing Engineering Technology Research Center of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou,P.R.China [2]Department of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou,PR.China [3]Guangdong Andao Medical Equipment Co.Ltd,Guangzhou,PR.China [4]Department of Pharmacy,Heilongjiang University of Chinese Medicine,Heilongjiang,PR.China [5]Macao Association of Chinese Medicine(AIPPMCM),Macao SAR,PR.China

出　　处：《World Journal of Traditional Chinese Medicine》2023年第2期167-177,共11页世界中医药杂志（英文）

基　　金：supported by the Guangzhou Science and Technology Program in 2020-Basic and Applied Basic Research Project(Grant No.202002030226);the National Natural Science Foundation of China(Grant No.82174043);the Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515011697).

摘　　要：Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory properties and can be used to treat acute lung injury and eczema.The aim of this study is to solve the problems of extraction and purification of active components from PAF. Materials and Methods: The solvent to be used for extraction and its concentration, the solid-to-liquid ratio, and extraction duration were investigated using a single-factor experiment.An orthogonal design(L_(9)[3^(4)]) was used to determine the optimum extraction conditions. After optimization, the sample’s concentrations and flow velocity, the eluents and their velocity, adsorption time, and the removed water volume were measured. The content of the five steroids in the sample was determined by high-performance liquid chromatography(HPLC). We also investigated the anti-inflammatory property of PAF calyxes before and after purification. Results: The optimum extraction and purification processes were determined by single-factor analysis.AB-8 was identified as the best macroporous adsorption resin for enrichment. After optimization, the average total steroid content was 71.83%,and the average recovery was 90% after purification. Among the five steroid components detected by HPLC, physalin F showed the highest content. Furthermore, the sample obtained after purification could significantly inhibit paw edema by egg whites induced. Conclusions: An environmentally-sustainable, efficient, and stable process was first optimized for enriching and purifying total steroids from PAF. The process has the potential for further development and utilization in the pharmaceutical industry.

关 键 词：Anti-inflammatory activity extraction and optimization process physalins Physalis alkekengi L.var.francheti(Mast.)Makino STEROIDS 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]