不同益生菌发酵复方中草药对芦丁含量的影响  

Effect of Fermentation by Different Probiotics on Content of Rutin in Compound Chinese Herbal Medicine

作　　者：田秋丰 张红 尹珺伊 张军 刘雪松 白长胜 王岩 秦平伟 王欢 刘秋瑾 朱庆贺 苗艳 江波涛 钟鹏 史同瑞 TIAN Qiu-feng;ZHANG Hong;YIN Jun-yi;ZHANG Jun;LIU Xue-song;BAI Chang-sheng;WANG Yan;QIN Ping-wei;WANG Huan;LIU Qiu-jin;ZHU Qing-he;MIAO Yan;JIANG Bo-tao;ZHONG Peng;SHI Tong-rui(Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Heilongjiang Provincial Key Laboratory of Veterinary Medicines,Qiqihar 161005,China)

机构地区：[1]黑龙江省农业科学院畜牧兽医分院、黑龙江省兽用药物重点实验室,黑龙江齐齐哈尔161005

出　　处：《中国兽医杂志》2023年第8期127-132,共6页Chinese Journal of Veterinary Medicine

基　　金：黑龙江省重点研发计划项目(GA21B006);黑龙江省农业科学院畜牧兽医分院2022年自拟项目(ZNKT-202205)。

摘　　要：为了提高复方中草药的提取率和利用率,增强药效和降低成本,考察不同益生菌发酵复方中草药对芦丁含量的影响,本试验利用干酪乳杆菌、解淀粉芽孢杆菌不同组合对复方中草药进行发酵,采用高效液相色谱法(HPLC)测定中草药发酵后芦丁的含量。结果显示,复方中草药经益生菌发酵后芦丁含量均有所提高,其中A组(解淀粉芽孢杆菌)芦丁含量均值为(1.33±0.05)mg/g,B组(解淀粉芽孢杆菌∶干酪乳酸菌=4∶1,V∶V)芦丁含量均值为(1.57±0.09)mg/g,C组(解淀粉芽孢杆菌∶干酪乳酸菌=1∶1,V∶V)芦丁含量均值为(2.01±0.05)mg/g,D组(解淀粉芽孢杆菌∶干酪乳酸菌=1∶4,V∶V)芦丁含量均值为(2.85±0.06)mg/g,而E组(对照组)芦丁含量均值为(1.24±0.07)mg/g。结果表明,复方中草药经益生菌发酵后能够提高芦丁含量,且使用解淀粉芽孢杆菌和干酪乳酸菌组成1∶4体积比混合发酵复方中草药时,芦丁含量最高。In order to improve extraction and utilization rates of compound Chinese herbal medicine,enhance efficacy and reduce cost,this study examined the effects of fermentation by different probiotics on the content of rutin in compound Chinese herbal medicine.Different combinations of Lactobacillus casei(L.casei)and Bacillus amyloliquefaciens(B.amyloliquefaciens)were used to ferment compound Chinese herbal medicine,and the content of rutin after fermentation was determined by high performance liquid chromatography(HPLC).The results showed that the content of rutin in compound Chinese herbal medicine increased after probiotic fermentation,and the mean content of rutin in group A(B.amyloliticus)was(1.33±0.05)mg/g,and that in group B(B.amyloliticus∶L.casaeus=4∶1,V∶V)was(1.57±0.09)mg/g,that in group C(B.amylolivier∶L.casei=1∶1,V∶V)was(2.01±0.05)mg/g,that in group D(B.amylolivier∶L.casei=1∶4,V∶V)was(2.85±0.06)mg/g,and that in group E(control group)was(1.24±0.07)mg/g.Thus,the rutin content in compound Chinese herbal medicine increased after probiotic fermentation,and the highest content of rutin was obtained after fermentation with the mixture of B.amyloliticus and L.casei at a V∶V ratio of 1∶4.

关 键 词：固体发酵 高效液相色谱 芦丁 

分 类 号：S853.7[农业科学—临床兽医学]