Evaluation of the effect of shodhana(detoxification)process using chromatographic profiling(HPTLC,HPLC,LC-MS,and GC-MS)and estimation of the content of toxic colchicine in Gloriosa superba Linn.tubers  

作　　者：Ajay Kumar Meena Poorna Venktaraman Mohit Motiwale Ravindra Singh Kusuma Ganji N.Srikanth K.S.Dhiman 

机构地区：[1]Regional Ayurveda Research Institute,Aamkho,Gwalior 474009,India [2]Captain Srinivasa Murthy Central Ayurveda Research Institute,Chennai 600106,India [3]Central Council for Research in Ayurvedic Sciences,Ministry of AYUSH,Govt.of India,New Delhi 110058,India

出　　处：《Asian Journal of Traditional Medicines》2023年第4期143-162,共20页亚洲传统医药（英文）

摘　　要：Shodhana(purification/detoxification)is an important aspect of Ayurveda.It has been described in several important pieces of literature.In this study,we performed shodhana process on Langali(Gloriosa superba Linn.)tubers of family Liliaceae,a semi-woody herbaceous climber with significant therapeutic properties.We compared the unprocessed(before purification)and processed(after purification)samples based on the phytochemical,physicochemical,and chromatographic study,including HPTLC,HPLC,GC-MS and LC-MS.Significant changes were observed in profiling after the shodhana process.This plant contains colchicine,known for its toxic effects,particularly cardiotoxicity.Therefore,we quantified colchicine in the unprocessed and processed samples of Gloriosa superba Linn.tubers using HPLC method.Results showed that the percentage of colchicine was reduced by 13.67%in chloroform extract and 20.97%in ethanol extracts after the shodhana process.

关 键 词：Shodhana HPLC LC-MS GC-MS COLCHICINE Gloriosa superba toxicity AYURVEDA 

分 类 号：R285[医药卫生—中药学]