桦褐孔菌醇提取物抗胃癌作用机制研究  

Anti-gastric Cancer Mechanism of Ethanol Extract of Inonotus obliquus

作　　者：乔羽[1] 郭亚迪 周忠光 刘旭 董霏雪[3] Qiao Yu;Guo Yadi;Zhou Zhongguang;Liu Xu;Dong Feixue(College of Basic Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Experimental Training Center,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Department of Ophthalmology,First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学基础医学院,哈尔滨150040 [2]黑龙江中医药大学实验实训中心,哈尔滨150040 [3]黑龙江中医药大学附属第一医院眼科,哈尔滨150040

出　　处：《肿瘤预防与治疗》2023年第8期623-630,共8页Journal of Cancer Control And Treatment

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划(编号:UPYSCT-2018231)。

摘　　要：目的:基于动物模型和细胞学实验,探讨桦褐孔菌醇提取物抗胃癌的作用机制。方法:将49只裸鼠随机分为7组:空白组(正常裸鼠)、模型组(植瘤鼠)、阴性对照组(只灌胃溶剂)、西药组(只灌卡培他滨)、高剂量组(桦褐孔菌醇提取液1.56 mg/g)、中剂量组(桦褐孔菌醇提取液0.78 mg/g)和低剂量组(桦褐孔菌醇提取液0.39 mg/g)。除空白组外,其它六组经腋下植瘤复制胃癌模型,灌胃给药21天。处死裸鼠,摘取瘤块,HE染色观察肿瘤组织的结构改变,免疫组化法测定Caspase-9、P53、Bcl-2及Bax的表达,ELISA法检测GSH-PX、SOD和MDA的含量。结果:高剂量组的肿瘤抑制率(36.77%)最高,其次是西药组(19.73%)、中剂量组(12.34%)和低剂量组(7.36%);西药组(0.64±0.08)g、高剂量组(0.50±0.11)g和中剂量组(0.70±0.77)g的瘤重与模型组(0.80±0.11)g瘤重相比,差异有统计学意义(均P<0.05);与模型组相比,桦褐孔菌醇提取物各剂量组细胞出现水肿坏死,有炎症浸润;Caspase-9、Bax、P53在西药组和高、中、低剂量组中蛋白表达上调,在模型组和阴性对照组表达下调,Bcl-2在高、中、低剂量组和西药组表达下调,模型组和阴性对照组表达上调;模型组MDA升高,与模型组比较,各治疗组表达均下调;模型组SOD、GSH-PX含量下降,与模型组比较,各治疗组表达均上调。结论:桦褐孔菌醇提取物对胃癌模型有明显的抑制作用,其作用机制与上调P53、Bax、Caspase-9,下调Bcl-2表达及抗氧化应激有关。Objective:The purpose of this experiment was to study the anti-cancer mechanism of Inonotus obliquus ethanol extract.Methods:Forty-nine nude mice were randomly divided into 7 groups:blank group(normal nude mice),model group(tumor-bearing mice),negative control group(only solvent by gavage),western medicine group(only capecitabine by gavage),highdose group(1.56 mg/g of Inonotus obliquus ethanol extract),middle-dose group(0.78 mg/g of Inonotus obliquus ethanol extract)and low-dose group(0.39 mg/g of Inonotus obliquus ethanol extract).In addition to the blank group,the other six groups replicated the gastric cancer model by tumor implantation in the axillary,and were given intragastric administration for 21 days.The nude mice were sacrificed and the tumor blocks were removed.The structural changes in tumor tissue were observed by HE staining.The expressions of caspase-9,P53,Bcl-2 and Bax were determined by immunohistochemistry.The content of GSH-Px,SOD and MDA were detected by ELISA.Results:The tumor inhibition rate in the high-dose group(36.77%)was the highest,followed by that in the western medicine group(19.73%),that in the middle-dose group(12.34%),and that in the low-dose group(7.36%).The tumor weights in the western medicine group(0.64±0.08),the high-dose group(0.50±0.11)and the middle-dose group(0.70±0.77)was significantly different from that in the model group(P<0.05).Compared with the model group,cells in the high-,middle-and low-dose groups showed edema,necrosis and inflammatory infiltration.Caspase-9,BAX and P53 were up-regulated in the western medicine group and the high-,middle-and low-dose groups,and down-regulated in the model group and the negative control group;while Bcl-2 was down-regulated in the the western medicine group and the high-,middle-and low-dose groups,and up-regulated in the model group and the negative control group.MDA content in the model group significantly increased,and was down-regulated after medication.The content of SOD and GSH-PX in the model group decreased significantly,

关 键 词：桦褐孔菌 胃癌 凋亡 氧化 

分 类 号：R735.2[医药卫生—肿瘤]