纤连蛋白Ⅲ型结构域蛋白5对HepG2肝癌细胞体外转移活性的影响及其机制  

Effect of fibronectin type III domain containing 5 on the metastatic activity of HepG2 hepatoma cells in vitro and its mechanism

作　　者：袁庆功[1] 张焱[1] 李军辉[1] 杨文彬[1] YUAN Qing-gong;ZHANG Yan;LI Jun-hui;YANG Wen-bin(Department of General Surgery,the Second Affiliated Hospital of Xi′an Jiaotong University,Shaanxi 710004,China)

机构地区：[1]西安交通大学第二附属医院普通外科,陕西710004

出　　处：《肝脏》2023年第8期943-946,共4页Chinese Hepatology

摘　　要：目的研究纤连蛋白Ⅲ型结构域蛋白5对肝细胞癌的影响以及调控机制。方法以不同浓度0、5、10和20μmol纤连蛋白Ⅲ型结构域蛋白5处理HeP G2细胞,通过MTT实验、集落形成实验、Transwell实验、流式细胞术和细胞划痕实验分析纤连蛋白Ⅲ型结构域蛋白5对HepG2细胞的活性、侵袭和迁移能力以及凋亡率的影响。通过蛋白免疫印迹法分析纤连蛋白Ⅲ型结构域蛋白对Wnt/β-catenin通路的作用。结果0、5、10和20μmol的纤连蛋白Ⅲ型结构域蛋白5可以降低HepG2细胞的生长活性(92.86±0.32比79.32±0.17比50.11±0.56比29.29±0.96),抑制HepG2细胞集落形成(238.25±12.28比126.91±9.56比68.93±9.25比32.53±7.32),并且能够阻止HepG2细胞的发生侵袭以及迁移(87.13±2.18比66.23±2.16比46.23±3.27比22.56±5.12;88.46±12.55比55.08±9.21比39.62±5.63比19.26±5.71),诱导HepG2细胞凋亡(1.46±0.23比5.08±0.11比9.66±0.23比13.27±1.01)。进一步研究发现,纤连蛋白Ⅲ型结构域蛋白5的这些作用可能与Wnt/β-catenin信号通路的抑制有关。结论纤连蛋白Ⅲ型结构域蛋白5能够抑制HepG2细胞的转移活性,并且纤连蛋白Ⅲ型结构域蛋白5的抑癌作用可能与Wnt/β-catenin途径有关。Objective To investigate the effect of FNDC5 on hepatocellular carcinoma(HCC)and its related mechanisms in order to propose new ideas for the treatment of HCC.Methods HepG2 cells were divided into four groups based on FNDC5 concentration(0,5,10 and 20μM).The activity,invasion and migration of HepG2 cells,as well as apoptosis rate,were analyzed by MTT test,colony formation test,Transwell test,flow cytometry and cell scratch test.The impact of FNDC5 on the Wnt/β-catenin pathway was analyzed by Western blot.Results FNDC5 reduced the growth activity of HepG2 cells(92.86±0.32 vs 79.32±0.17 vs 50.11±0.56 vs 29.29±0.96),inhibited HepG2 cell colony formation(238.25±12.28 vs 126.91±9.56 vs 68.93±9.25 vs 32.53±7.32),and prevented the occurrence of HepG2 cell invasion and migration(87.13±2.18 vs 66.23±2.16 vs 46.23±3.27 vs 22.56±5.12;88.46±12.55 vs 55.08±9.21 vs 39.62±5.63 vs 19.26±5.71).FNDC5 also induced apoptosis in HepG2 cells(1.46±0.23 vs 5.08±0.11 vs 9.66±0.23 vs 13.27±1.01).Conclusion HepG2 cell metastatic activity can be inhibited by FNDC5,and the anti-tumor effect of FNDC5 may be connected to the Wnt/β-catenin signal pathway.

关 键 词：纤连蛋白Ⅲ型结构域蛋白5 HEPG2 转移活性 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]