马铃薯PTST1基因的克隆、表达及互作分析  

Cloning of PTST1 and analysis of its expression and interaction in potato

作　　者：石振明 杨慧芹[1] 高冬丽 SHI Zhenming;YANG Huiqin;GAO Dongli(Key Lab of Yunnan Province for Biomass Energy and Environmental Biotechnology,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学云南省生物质能与环境生物技术重点实验室,昆明650500

出　　处：《植物生理学报》2023年第8期1575-1582,共8页Plant Physiology Journal

基　　金：国家自然科学基金地区科学基金(32060684);云南师范大学博士启动项目(2021ZB005);云南省院士工作站(202105AF150028)。

摘　　要：马铃薯是世界上主要粮食作物之一,其块茎中的贮藏淀粉是马铃薯的主要利用产物。PROTEIN TARGETING TO STARCH 1(PTST1)基因在直链淀粉合成中具有重要作用。然而,它在马铃薯中的功能尚不清楚。本文以马铃薯PTST1为目的基因,从基因特征、表达模式及与GBSSI的互作关系方面进行了研究。PTST1蛋白由252个氨基酸残基组成,羧基端含有碳水化合物结合结构域。PTST1在多个组织的表达均低于GBSSI,在块茎发育时期,其表达没有明显的升高趋势。GBSSI在块茎中高表达,且随着块茎发育,其表达升高,说明PTST1与GBSSI的表达模式不同。酵母双杂交实验、烟草荧光素酶互补实验和双分子荧光互补实验证明PTST1与GBSSI蛋白互作,且互作发生于叶绿体基质。上述结果表明PTST1可能与叶片和块茎中直链淀粉的合成有密切的关系。本研究为调控马铃薯直链淀粉合成提供了新的基因资源。Potato(Solanum tuberosum)is one of the main food crops in the world,and storage starch in the tubers is the main processed product.PROTEIN TARGETING TO STARCH 1(PTST1)gene plays an important role in the synthesis of amylose.However,its function in potato has not been elucidated.In this study,potato PTST1 was studied in terms of its molecular characteristics,expression pattern and its interaction with GBSSI.Potato PTST1 protein is comprised of 252 amino acid residues and contains a carohydrate-binding module(CBM)at its C-terminus.The transcript level of PTST1 was lower than that of GBSSI in multiple tissues,and was not considerably increased during tuber development.GBSSI was highly expressed in tubers,and was generally increased during tuber development,suggesting that two genes have different expression pattern.The yeast two-hybrid experiments,firefly luciferase complementation assays and bimolecular fluorescent complimentary assays proved that PTST1 could interact with GBSSI,and the interaction occured in the chroloplast stroma.The results indicate that PTST1 protein may be closely associated with amylose biosynthesis in leaves and tubers.This study provides a new gene resource for regulating amylose biosynthesis of potato.

关 键 词：马铃薯 淀粉合成 PTST1 GBSSI 

分 类 号：S532[农业科学—作物学]