耐酸青霉菌Penicillium purpurogenum OUCMDZ-019的次级代谢产物研究  

Study on secondary metabolites of acid resistant Penicillium purpurogenum OUCMDZ-019

作　　者：王雨晴 于登 侯成凤 付鹏 朱伟明[1,2] 王乂 WANG Yuqing;YU Deng;HOU Chengfeng;FU Peng;ZHU Weiming;WANG Yi(School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学医药学院,山东青岛266003 [2]青岛海洋科学与技术国家实验室海洋药物与生物制品功能实验室,山东青岛266237

出　　处：《中国海洋药物》2023年第4期8-12,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81973198,82111540166,U1906213)资助。

摘　　要：目的对耐酸产紫青霉OUCMDZ-019的次级代谢产物进行化学成分和生物活性研究,发现潜力结构资源。方法对菌株进行规模发酵得到粗提物,利用常压柱层析、凝胶分子筛以及半制备高效液相等方法进行次级代谢产物的分离纯化;利用现代波谱学方法(核磁共振1D NMR、2D NMR、圆二色谱ECD、旋光等)对化合物进行结构鉴定,并评价其抗病毒生物活性。结果分离鉴定得到4个单体化合物,(-)-mitorubrinol(1)、6″-Hydroxy-(R)-mitorubrinic acid(2)、(-)-mitorubrinic acid(3)、purpurquinone D(4)。结论从耐酸真菌产紫青霉OUCMDZ-019的次级代谢产物中分离鉴定得到了化合物1~4,均属于嗜氮酮类化合物。生物活性测试结果显示,化合物1~4均无显著抗H1N1病毒活性。研究获得量产化合物3,为后续药物的结构衍生打下基础。Objective To study the secondary metabolites of acid resistant fungus Penicillium purpurogenum OUCMDZ-019.Methods The crude extracts were obtained by large-scale fermentation.The secondary metabolites were separated by column chromatography,gel molecular sieve and semi high performance liquid chromatography.The structures of the compounds were identified by modern spectroscopy methods(nuclear magnetic resonance 1D NMR,2D NMR,circular dichroism ECD,optical rotation,etc.).Results Four compounds were isolated and identified as(-)-mitorubinol(1),6″-Hydroxy-(R)-mitorubrinic acid(2),(-)-mitorubrinic acid(3) and purpurquinone D(4).Conclusion Compounds 1-4 were isolated and identified from the secondary metabolites of acid resistant fungus Penicillium purpurogenum OUCMDZ-019,which belong to azaphilones.Compounds 1-4 have no significant anti H1N1 virus activityies.The large-scale mass production of compound 3 was obtained,which made a base for the structural derivation of subsequent drugs.

关 键 词：耐酸真菌 嗜氮酮类化合物 产紫青霉 

分 类 号：R914[医药卫生—药物化学]