苦水玫瑰急性经口毒性和遗传毒性研究  

Study on acute oral toxicity and genotoxicity of Rosa sertata×Rosa rugosa Yu.et.Ku.

作　　者：丁小全[1] 魏琳琳[1] 冯文燕 王炯蓉[1] 许小红[1] 张东城 宋伟忠 孙建云[1] 何晓军[1] DING Xiaoquan;WEI Linlin;FENG Wenyan;WANG Jiongrong;XU Xiaohong;ZHANG Dongcheng;SONG Weizhong;SUN Jianyun;HE Xiaojun(Toxicology Department of Gansu Provincial Center for Disease Control and Prevention,Lanzhou,Gansu,730020,China)

机构地区：[1]甘肃省疾病预防控制中心毒理室,甘肃兰州730020

出　　处：《甘肃中医药大学学报》2023年第4期1-7,共7页Journal of Gansu University of Chinese Medicine

基　　金：甘肃省中医药科研项目(GZK-2019-61)。

摘　　要：目的观察苦水玫瑰急性经口毒性与遗传毒性,为评价其食用安全性提供实验依据。方法急性经口毒性试验采用限量法,选择SPF级昆明小鼠20只,雌雄各半,苦水玫瑰日累积给药剂量为10.8 g/kg,记录小鼠的一般行为、中毒表现、死亡情况及小鼠体质量,并计算半数致死量(LD_(50))。鼠伤寒沙门氏菌回复突变试验(Ames试验)采用平板掺入法,选用TA97a、TA98、TA100、TA102、TA1535等5株鼠伤寒沙门氏菌突变型菌株,在有或无代谢活化系统(S9)的条件下分别进行试验,计数各组每皿回变菌落数。小鼠骨髓嗜多染红细胞微核试验采用30 h给受试物法,选择SPF级昆明小鼠50只,雌雄各半,随机分为5组,即苦水玫瑰高、中、低剂量组,空白对照组,阳性对照组,每组10只,分别灌胃相应的受试物或阳性物,于第2次灌胃6 h后处死小鼠,取股骨骨髓制片,计数各组出现微核的嗜多染红细胞并计算微核率。小鼠精母细胞染色体畸变试验选择SPF级昆明雄性小鼠25只,随机分为苦水玫瑰高、中、低剂量组,空白对照组,阳性对照组5组,每组5只,除阳性对照组腹腔注射环磷酰胺外,其他组分别灌胃相应的受试物,共给药5 d,第13天各组小鼠腹腔注射秋水仙素(4 mg/kg),4 h后处死,取两侧睾丸分离精母细胞制片,油镜阅片分析,计算小鼠精母细胞染色体畸变发生率。结果小鼠急性经口毒性试验苦水玫瑰剂量达10.8 g/kg时,观察期内无小鼠死亡,LD_(50)大于10.8 g/kg;苦水玫瑰对鼠伤寒沙门氏菌TA97a、TA98、TA100、TA102、TA1535等5株试验菌株,在有或无S9时,回变菌落数均未超过自发回变组的2倍,无剂量-反应关系,亦无可重复的并有统计学意义的阳性反应的测试点;与空白对照组比较,苦水玫瑰各剂量组微核细胞发生率、嗜多染红细胞/红细胞、精母细胞染色体畸变率无明显变化,差异均无统计学意义(P>0.05)。结论依急性毒性剂量分级标准,苦水玫Objective To observe the acute oral toxicity and genotoxicity of Rosa sertata×Rosa rugosa Yu.et.Ku.,and to provide experimental evidence for evaluating its edible safety.Methods Twenty SPF Kunming mice,half male and half female,were selected for acute oral toxicity test.The cumulative daily dose of Rosa sertata×Rosa rugosa Yu.et.Ku.was 10.8 g/kg.The general behavior,toxicity,death and body mass of the mice were recorded,and LD_(50)was calculated.Five mutant strains of Salmonella Typhimurium,including TA97a,TA98,TA100,TA102 and TA1535,were selected for the Ames test with or without metabolic activation system(S9),and the number of reverted colonies per dish in each group was counted.Mice bone marrow polychromic erythrocyte micronucleus test was performed for 30 h.50 SPF Kunming mice,half male and half female,were selected and randomly divided into 5 groups,namely high-dose,medium-dose and low-dose groups of Rosa sertata×Rosa rugosa Yu.et.Ku.,blank control group and positive control group,with 10 mice in each group,respectively.The mice were killed after the second gavage for 6 h.The bone marrow of femur was dissected,polychromatic erythrocytes with micronucleus in each group were counted and micronucleus rate was calculated.Twenty-five SPF Kunming male mice were randomly divided into high,medium and low dose groups,blank control group and positive control group with 5 mice in each group.Except the positive control group,cyclophosphamide was injected intraperitoneally,and the other groups were administrated with corresponding test subjects for a total of 5 days.On the 13th day,mice in each group were injected intraperitoneally with colchicine(4 mg/kg).4 h later,the mice were killed,and spermatocytes were separated from both testis for production,and the incidence of chromosome aberration in spermatocytes was calculated.Results When the acute oral toxicity test dose of Rosa sertata×Rosa rugosa Yu.et.Ku.of mice reached 10.8 g/kg,no mice died during the observation period,and the LD_(50)was greater than 10.8 g/kg.

关 键 词：苦水玫瑰 急性经口毒性 遗传毒性 食品安全 实验研究 

分 类 号：R285.5[医药卫生—中药学]