IL-34介导的NF-кB信号通路对根尖牙乳头干细胞增殖、迁移的调节作用  

Regulation of IL-34-mediated NF-кB signaling pathway on proliferation and migration of stem cells from apical papilla

作　　者：武峻捷 朱永娜 姜丽娜 武庆华[1] 张伟健 石昕 刘茜[1] WU Jun-jie;ZHU Yong-na;JIANG Li-na;WU Qing-hua;ZHANG Wei-jian;SHI Xin;LIU Xi(Department of Stomatology,The Second Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233040;School of Stomatology,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院第二附属医院口腔科,安徽蚌埠233040 [2]蚌埠医学院口腔医学院,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2023年第8期1024-1029,共6页Journal of Bengbu Medical College

基　　金：蚌埠医学院自然科学研究重点项目(2021byzd192);蚌埠医学院研究生创新创业自然科学项目(Byycx22146)。

摘　　要：目的:探讨白细胞介素-34(IL-34)能否通过调控核因子-кB(NF-кB)影响根尖牙乳头干细胞(SCAPs)增殖、迁移。方法:取小鼠中切牙根尖牙乳头组织,酶消化法传代培养SCAPs;流式细胞术鉴定SCAPs表面CD44、CD45、CD90以及CSF-1R的表达;将SCAPs分为4组:空白对照组、IL-34组(100 ng/mL IL-34)、CSF-1R组(100 ng/mL IL-34+25 ng/mL anti-CSF-1R)和NF-кB组(100 ng/mL IL-34+1μmol/L BMS-345541);Western blotting分析各组中,30、60、120 min时SCAPs细胞质、细胞核中p-IкBα、IкBα、p-P65及p65蛋白的相对表达量;Transwell实验分析各组SCAPs迁移能力;将每组IL-34更换为50 ng/mL,CCK-8分析各组SCAPs增殖变化情况。结果:流式细胞术鉴定SCAPs表达CD44、CD90阳性,CD45阴性;SCAPs表面表达CSF-1R;与对照组SCAPs相比,IL-34处理后的SCAPs细胞质、细胞核内p-IкBα、p-P65与细胞质、细胞核中p-P65相对表达量显著增加,SCAPs增殖、迁移能力增强。与IL-34组相比CSF-1R组、NF-кB组内SCAPs增殖、迁移能力降低,SCAPs细胞质、细胞核中p-IкBα、p-P65与胞核中p-P65蛋白表达明显减少。结论:IL-34可调节SCAPs中NF-кB信号通路,并通过NF-кB信号通路调节IL-34的增殖和迁移。Objective:To investigate whether interleukin-34(IL-34)can affect the proliferation and migration of stem cells from apical papilla(SCAPs)by the regulation of nuclear factor-κB(NF-кB).Methods:The enzymatic digestion method was used to culture SCAPs from the apical papillae of mice with medium incisors.The expression of CD44,CD45,CD90 and CSF-1R on the surface of SCAPs was identified by flow cytometry.SCAPs were divided into 4 groups including blank control group,IL-34 group(100 ng/mL IL-34),CSF-1R group(100 ng/mL IL-34+25 ng/mL anti-CSF-1R)and NF-κB group(100 ng/mL IL-34+1μmol/L BMS-345541).Western blotting was used to analyze the relative expression of p-IкBα,IкBα,p-P65 and P65 protein in the cytoplasm and nucleus of SCAPs at 30,60 and 120 minutes in each group.Transwell assay was performed to analyze the migration ability of SCAPs in each group.IL-34 concentration was changed to 50 ng/mL in each group,and CCK-8 was used to analyze the change of proliferation of SCAPs in each group.Results:Flow cytometry identified that SCAPs expressed CD44,CD90 positive and CD45 negative,and CSF-1R expressed on the surface of SCAPs.Compared with the control group,the relative expression of p-IкBα,p-P65 in the cytoplasm and p-P65 in the nucleus of SCAPs treated with IL-34 was significantly increased and the proliferation and migration ability of SCAPs were enhanced.Compared with the IL-34 group,the proliferation and migration of SCAPs were decreased in the CSF-1R and NF-кB group,and the expression of p-IкBα,p-P65 in the cytoplasm and p-P65 protein in the nucleus of SCAPs was significantly reduced.Conclusions:IL-34 can regulate NF-κB signaling pathway in SCAPs and regulate the proliferation and migration of IL-34 through NF-κB signaling pathway.

关 键 词：根尖牙乳头干细胞 白细胞介素-34 增殖 迁移 核因子-кB信号通路 

分 类 号：R78[医药卫生—口腔医学]