A novelα-ketoamide reactivity-based two-photon fluorogenic probe for visualizing peroxynitrite in Parkinson's disease models  

作　　者：Tao Shao Xianning Xu Lan Wang Yu Shen Jun Zhao Huizi Li Duoteng Zhang Wei Du Hua Bai Bo Peng Lin Li 

机构地区：[1]Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials&Engineering,Northwestern Polytechnical University Xi'an 710072,P.R.China [2]Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM)Nanjing Tech University(NanjingTech),Nanjing 211816,P.R.China [3]Department of Outpatient PLA Rocket Force Characteristic Medical Center Beijing 100088,P.R.China [4]The Institute of Flexible Electronics(IFE,Future Technologies)Xiamen University,Xiamen 361005,Fujian,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2023年第4期79-89,共11页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(22077101);the Open Project Program of Wuhan National Laboratory for Optoelectronics(No.2020WNLOKF023);Natural Science Foundation of Shaanxi Province(2022JM-130);the Joint Research Funds of Department of Science&Technology of Shaanxi Province and Northwestern Polytechnical University(2020GXLH-Z-008,2020GXLH-Z-021,2020GXLH-Z-023);the China Postdoctoral Science Foundation(2022M711595,2022M722595);Postdoctoral Research Funding Schemes of Jiangsu Province(2021K036A);The Natural Science Foundation of Ningbo(202003N4049,202003N4065).

摘　　要：Peroxynitrite(ONOO^(-))contributes to oxidative stress and neurodegeneration in Parkinson's disease(PD).Developing a peroxynitrite probe would enable in situ visualization of the overwhelming ONOO^(-)flux and understanding of the ONOO^(-)stress-induced neuropathology of PD.Herein,a novelα-ketoamide-based fluorogenic probe(DFlu)was designed for ONOO^(-)
monitoring in multiple PD models.The results demonstrated that DFlu exhibits a fluorescence turn-on response to ONOO^(-)with high specificity and sensitivity.The efficacy of DFlu for intracellular ONOO^(-)
imaging was demonstrated systematically.The results showed that DFlu can successfully visualize endogenous and exogenous ONOO^(-)in cells derived from chemical and biochemical routes.More importantly,the two-photon excitation ability of DFlu has been well demonstrated by monitoring exogenous/endogenous ONOO^(-)production and scavenging in live zebraflsh PD models.This work provides a reliable and promising
α-ketoamide-based optical tool for identifying variations of ONOO^(-)in PD models.

关 键 词：α-Ketoamide two-photon fluorogenic probe BIOIMAGING PEROXYNITRITE Parkinson's disease. 

分 类 号：R318[医药卫生—生物医学工程] R742.5[医药卫生—基础医学]