电针改善糖尿病肾病模型小鼠肾微血管损伤  

Mitigation of renal microvascular injury in diabetic kidney disease model mice by electroacupuncture

作　　者：向继琴 魏文静 廖美华 张可兴 周颖琪 张文捷 唐纯志[1,2] 许能贵 陶蓉蓉[1,2] Ji-qin XIANG;Wen-jing WEI;Mei-hua LIAO;Ke-xing ZHANG;Ying-qi ZHOU;Wen-jie ZHANG;Chun-zhi TANG;Neng-gui XU;Rong-rong TAO(Medical College of Acupuncture-Moxibustion and Rehabilitation,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;South China Research Center for Acupuncture and Moxibustion,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学针灸康复临床医学院,广州510006 [2]广州中医药大学华南针灸研究中心,广州510006

出　　处：《World Journal of Acupuncture-Moxibustion》2023年第3期262-272,共11页世界针灸杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China:82274628;Natural Science Foundation of Guangdong Province:2023A1515030167;Discipline Collaborative Innovation Team Program of Double First-class and High-level Universities for Guangzhou University of Chinese Medicine:2021XK01。

摘　　要：Objective:Renal microvascular injury,as the result of diabetic toxicity,plays a vital role in the pathogenesis of diabetic kidney disease(DKD)during diabetes progression.Here,we investigated whether electroacupuncture(EA)could ameliorate renal microvascular impairment to prevent DKD and its underlying mechanism.Methods:The male db/db mice with Leprdb mutation were used as the model of type 2 diabetes mellitusinduced DKD and treated with EA at"Zusanli(ST36)"and"Weiwanxiashu(EX-B3)"acupoints for 4 weeks.Age-matched wild-type mice were used as control group.Renal protection of EA was evaluated by mouse urine production,water consumption,renal index and tubules dilation.Two-photon microscope imaging was applied to visualize renal microvascular blood flow in vivo.Immunostaining and western blot analysis were used to detect the glomerular alternations and inflammatory signaling.Results:EA significantly attenuated renal dysfunction in db/db mice.The protective effect of EA on renal microvascular recovery was observed both in function and structure analysis.Firstly,EA restored the renal microvascular blood flow in db/db mice.Then,glomerular hypertrophy and glomerular barrier destruction were suppressed after EA,as respectively demonstrated by the reduction of glomerular dilation,Collagen IV and Claudin-1 deposits.In mechanism,EA suppressed the diabetes-induced inflammatory response in renal microvessels,presenting as the downregulation of inflammatory cytokines interleukin-1β(IL-1β)and tumor necrosis factor(TNF-α),intercellular cell adhesion molecule-1(ICAM-1)activation,and macrophage infiltration after EA treatment.Conclusion:These findings indicated the benefits of EA against renal microvascular impairment and DKD progression,which was associated with the action of anti-inflammation,and supported EA as a promising modality for DKDmanagement.

关 键 词：Diabetic kidney disease ELECTROACUPUNCTURE ACUPUNCTURE Renal microvascular injury INFLAMMATION Two-photon microscope imaging 

分 类 号：R245.97[医药卫生—针灸推拿学]