Translocation of telomerase reverse transcriptase coincided with ATP release in postnatal cochlear supporting cells  

作　　者：Yukai Zhang Keyong Tian Wei Wei Wenjuan Mi Fei Lu Zhenzhen Liu Qingwen Zhu Xinyu Zhang Panling Geng Jianhua Qiu Yongli Song Dingjun Zha 

机构地区：[1]Department of Otolaryngology,Xijing Hospital,Fourth Military Medical University,Xi’an,Shaanxi Province,China

出　　处：《Neural Regeneration Research》2024年第5期1119-1125,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.81870732(to DZ),82171161(to DZ),81900933(to YS),and 82000978(to ZL).

摘　　要：The spontaneous bursts of electrical activity in the developing auditory system are derived from the periodic release of adenosine triphosphate(ATP)by supporting cells in the Kölliker’s organ.However,the mechanisms responsible for initiating spontaneous ATP release have not been determined.Our previous study revealed that telomerase reverse transcriptase(TERT)is expressed in the basilar membrane during the first postnatal week.Its role in cochlear development remains unclear.In this study,we investigated the expression and role of TERT in postnatal cochlea supporting cells.Our results revealed that in postnatal cochlear Kölliker’s organ supporting cells,TERT shifts from the nucleus into the cytoplasm over time.We found that the TERT translocation tendency in postnatal cochlear supporting cells in vitro coincided with that observed in vivo.Further analysis showed that TERT in the cytoplasm was mainly located in mitochondria in the absence of oxidative stress or apoptosis,suggesting that TERT in mitochondria plays roles other than antioxidant or anti-apoptotic functions.We observed increased ATP synthesis,release and activation of purine signaling systems in supporting cells during the first 10 postnatal days.The phenomenon that TERT translocation coincided with changes in ATP synthesis,release and activation of the purine signaling system in postnatal cochlear supporting cells suggested that TERT may be involved in regulating ATP release and activation of the purine signaling system.Our study provides a new research direction for exploring the spontaneous electrical activity of the cochlea during the early postnatal period.

关 键 词：apoptosis ATP release Ca2+transients COCHLEA mitochondrial function reactive oxygen species spontaneous electrical activity supporting cells 

分 类 号：R764[医药卫生—耳鼻咽喉科]