基于Heracles NEO超快速气相电子鼻麸炒白术炮制全过程气味变化识别研究  

Identification of odor changes in whole process of Atractylodis Macrocephalae Rhizoma processing based on Heracles NEO ultra-fast gas phase electronic nose

作　　者：李亚飞 汤璐璐 赵明方 唐瑞 方可儿 张海瑞 杜伟锋[1,2,3] 葛卫红 LI Ya-fei;TANG Lu-lu;ZHAO Ming-fang;TANG Rui;FANG Ke-er;ZHANG Hai-rui;DU Wei-feng;GE Wei-hong(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 311402,China;Research Center of TCM Processing Technology,Zhejiang Chinese Medical University,Hangzhou 311401,China;Zhejiang Chinese Medical University Chinese Medicine Yinpian Co.,Ltd.,Hangzhou 311401,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311402 [2]浙江中医药大学中药炮制技术研究中心,浙江杭州311401 [3]浙江中医药大学中药饮片有限公司,浙江杭州311401

出　　处：《中草药》2023年第15期4812-4822,共11页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划—中药饮片质量识别关键技术研究(2018YFC1707001);杭州市农业与社会发展科研重点项目(202204A06);宁波市“科技创新2025”重大专项(2020Z089)。

摘　　要：目的建立麸炒白术Atractylodis Macrocephalae Rhizoma炮制全过程的定性判别模型,筛选出可以表征麸炒白术不同炮制程度的气味成分。方法制备麸炒白术饮片,不同时间点取样进行分析。采用Heracles NEO超快速气相电子鼻采集气味图谱,与Arochembase数据库对比得到气味成分信息,结合峰面积分析气味成分的变化规律并进行化学计量学分析。结果通过判别因子分析(discriminant factor analysis,DFA)、正交偏最小二乘-判别分析(orthogonal partial least squaresdiscriminant analysis,OPLS-DA)、层次聚类分析(hierarchical cluster analysis,HCA)等将麸炒白术炮制全过程样品成功分为4类:生品、炮制不及、炮制成品和炮制太过。在炮制过程中,3-甲基十五烷、十二烷基丙酸酯、正辛基环己烷、1,3-二硝基苯、8-甲基十五烷5个气味成分的峰面积占比较高,其中1,3-二硝基苯在炮制全过程中峰面积呈降低趋势;3-甲基十五烷从炮制不及到炮制成品的过程中峰面积呈增加的趋势,从炮制成品到炮制太过的过程中峰面积呈降低趋势;十二烷基丙酸酯从生品到炮制不及和炮制成品到炮制太过的过程中峰面积呈降低趋势;正辛基环己烷、8-甲基十五烷从生品到炮制不及的过程中峰面积呈降低趋势,从炮制不及到炮制成品的过程中峰面积呈增加趋势,从炮制成品到炮制太过的过程中峰面积呈降低趋势,且上述5个气味成分的变量重要性投影值均大于1。结论Heracles NEO超快速气相电子鼻能通过气味对麸炒白术不同炮制程度样品进行定性判别,3-甲基十五烷、十二烷基丙酸酯、正辛基环己烷、1,3-二硝基苯及8-甲基十五烷5个气味成分的变化可作为麸炒白术不同炮制程度的判断依据。Objective Establish a qualitative discriminant model for the whole process of Baizhu(Atractylodis Macrocephalae Rhizoma,AMR)stir-fried with wheat bran(wbAMR),and screen out the odor components that can characterize the different processing degrees wbAMR.Methods AMR decoction pieces with different processing time were prepared by wheat bran stir-frying method.The odor spectrum was collected by ultra-high-speed gas phase electronic nose,and the odor component information was obtained by comparing with the Arochembase database.The variation of odor components was analyzed by peak area and chemometrics analysis was carried out.Results Through discriminant factor analysis(DFA),orthogonal partial least squares-discriminant analysis(OPLS-DA),hierarchical cluster analysis(HCA),etc.,the whole process samples of wbAMR were successfully divided into four categories:raw products,less processed,processed products and processed too much.The peak area of 1,3-dinitrobenzene showed a decreasing trend during the whole processing process.The peak area of 3-methylpentadecane showed an increasing trend from raw products to less processed,and the peak area showed a decreasing trend from processed products to processed too much.The peak area of dodecyl propionate decreased from raw products to less processed and from processed products to processed too much.The peak area of n-octylcyclohexane and 8-methylpentadecane showed a decreasing trend from raw products to less processed products,an increasing trend from less processed products to processed products,and a decreasing trend from processed products to processed too much.And the variable importance projection values of the above five odor components were all greater than 1.Conclusion Heracles NEO ultra-fast gas phase electronic nose can qualitatively distinguish the samples of different processing degrees of wbAMR by odor.The changes of 5 odor components of 3-methylpentadecane,dodecyl propionate,n-octylcyclohexane,1,3-dinitrobenzene and 8-methylpentadecane can be used as the basis fo

关 键 词：麸炒白术 超快速气相电子鼻 定性判别 炮制过程 气味成分 3-甲基十五烷 十二烷基丙酸酯 正辛基环己烷 1 3-二硝基苯 8-甲基十五烷 判别因子分析 正交偏最小二乘-判别分析 层次聚类分析 

分 类 号：R283.6[医药卫生—中药学]