SETD1A与KDM4A在非小细胞肺癌中的表达及临床价值  

The expression and clinical value of SETD1A and KDM4A in non-small cell lung cancer

作　　者：朱小慧[1] 李亚云[1] 张海燕[2] 刘海涛[1] 于志娟[1] Zhu Xiaohui;Li Yayun;Zhang Haiyan;Liu Haitao;Yu Zhijuan(Department of General Internal Medicine,Nantong Tumor Hospital,Jiansu Province,Nantong 226001,China)

机构地区：[1]江苏省南通市肿瘤医院综合内科,226001 [2]江苏省南通市肿瘤医院放疗科,226001

出　　处：《疑难病杂志》2023年第9期945-950,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：江苏省卫生健康委基金资助项目(JH19044)。

摘　　要：目的研究非小细胞肺癌(NSCLC)组织中组蛋白赖氨酸甲基转移酶SET结构域包含1A(SETD1A)和赖氨酸去甲基酶4A(KDM4A)表达及与临床病理特征及预后的关系。方法选取2017年1月—2020年1月南通市肿瘤医院综合内科收治NSCLC患者116例为研究对象。采用免疫组织化学法检测NSCLC组织中SETD1A、KDM4A表达,二者相关性分析采用Spearman秩相关分析。Kaplan-Meier生存分析SETD1A、KDM4A表达对NSCLC患者生存预后的影响。Cox回归分析影响NSCLC患者预后因素。结果NSCLC癌组织中SETD1A棕黄色阳性染色主要位于细胞核,KDM4A阳性染色主要位于细胞浆和细胞膜。NSCLC癌组织SETD1A阳性率为61.21%(71/116),高于癌旁组织的6.90%(8/116),差异有统计学意义(χ^(2)=76.546,P<0.001)。NSCLC癌组织中KDM4A阳性率为65.52%(76/116),高于癌旁组织的8.62%(10/116),差异有统计学意义(χ^(2)=80.487,P<0.001)。NSCLC癌组织中SETD1A与KDM4A表达呈显著正相关(rs=0.722,P<0.001)。TNM分期Ⅲ期、低分化程度、伴淋巴结转移患者在NSCLC癌组织中SETD1A、KDM4A阳性率显著高于TNM分期Ⅰ~Ⅱ期、高中分化程度、无淋巴结转移者(SETD1A:χ^(2)/P=15.808/<0.001,7.108/0.008,17.136/<0.001;KDM4A:χ^(2)/P=9.050/0.003,5.480/0.019,10.208/0.001)。SETD1A阳性组和阴性组患者3年总体生存率分别为49.28%(34/69)、78.72%(37/47)。SETD1A阳性组患者3年累积生存率明显低于阴性组患者(χ^(2)=12.984,P<0.001);KDM4A阳性组和阴性组3年总体生存率分别为48.65%(36/74)、83.33%(35/42),KDM4A阳性组患者3年累积生存率明显低于阴性组患者(χ^(2)=17.175,P<0.001)。TNM分期Ⅲ期、合并淋巴结转移、SETD1A阳性、KDM4A阳性是影响NSCLC患者不良预后的独立危险因素[OR(95%CI)=1.610(1.221~2.122)、1.904(1.307~2.774)、1.835(1.228~2.742)、1.744(1.245~2.443)]。结论NSCLC癌组织中SETD1A、KDM4A表达升高,两者表达与TNM分期、分化程度及淋巴结转移有关,检测NSCLC组织中SETD1A、KDM4A表达有助�Objective To study the expression of histone lysine methyltransferase SET domain including 1A(SETD1A)and lysine Nor-4A(KDM4A)in non-small cell lung cancer(NSCLC)and its relationship with clinicopathological characteristics and prognosis.Methods One hundred and sixteen NSCLC patients admitted to the General Department of Nantong Cancer Hospital from January 2017 to January 2020 were selected as the study subjects.Immunohistochemical method was used to detect the expression of SETD1A and KDM4A in NSCLC tissue,and Spearman rank correlation analysis was used to analyze their correlation.Kaplan Meier survival analysis of the impact of SETD1A and KDM4A expression on the survival prognosis of NSCLC patients.Cox regression analysis of prognostic factors affecting NSCLC patients.Results In NSCLC cancer tissue,SETD1A brown yellow positive staining is mainly located in the nucleus,while KDM4A positive staining is mainly located in the cytoplasm and cell membrane.The positive rate of SETD1A in NSCLC cancer tissue was 61.21%(71/116),which was higher than 6.90%(8/116)in adjacent tissues(χ^(2)=76.546,P<0.001).The positive rate of KDM4A in NSCLC cancer tissue was 65.52%(76/116),higher than 8.62%(10/116)in adjacent tissues(χ^(2)=80.487,P<0.001).The expression of SETD1A and KDM4A in NSCLC cancer tissue showed a significant positive correlation(rs=0.722,P<0.001).The positive rates of SETD1A and KDM4A in NSCLC cancer tissue were higher in patients with TNM stage III,low differentiation,and lymph node metastasis than in patients with TNM stage I-II,high differentiation,and no lymph node metastasis(SETD1A:χ^(2)/P=15.808/<0.001,7.108/0.008,17.136/<0.001;KDM4A:χ^(2)/P=9.050/0.003,5.480/0.019,10.208/0.001).The overall 3-year survival rates of patients in the SETD1A positive and negative groups were 49.28%(34/69)and 78.72%(37/47),respectively.The 3-year cumulative survival rate of patients in the SETD1A positive group is significantly lower than that of patients in the negative group(χ^(2)=12.984,P<0.001);The overall 3-year survival

关 键 词：非小细胞肺癌 组蛋白赖氨酸甲基转移酶SET结构域包含1A 赖氨酸去甲基酶4A 预后 

分 类 号：R734.2[医药卫生—肿瘤]