调整FUT8表达对食管鳞癌细胞放射敏感性的影响  

To investigate effect of regulating FUT8 expression on radiosensitivity of esophageal squamous cell carcinoma cells

作　　者：刘永宇 夏敏 唐园慧 汪诗琪 骆志国 HU Qing LIU Yongyu;XIA Min;HU Qing;TANG Yuanhui;WANG Shiqi;LUO Zhiguo(Postgraduate Training Base of Jinzhou Medical University in Shiyan Taihe Hospital,Shiyan,Hubei 442000,China;Cancer Prevention and Treatment Center,Taihe Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China;Department of Oncology,Wuhan People's Hospital,Wuhan,Hubei 430000,China;Graduate School of Hubei University of Medicine,Shiyan,Hubei 442000,China;Department of Nephrology,Taihe Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]锦州医科大学十堰市太和医院研究生培养基地,湖北十堰442000 [2]湖北医药学院附属医院十堰市太和医院肿瘤防治中心,湖北十堰442000 [3]武汉市人民医院肿瘤科,湖北武汉430000 [4]湖北医药学院研究生院,湖北十堰442000 [5]湖北医药学院附属医院十堰市太和医院肾内科,湖北十堰442000 [6]不详

出　　处：《中国医学工程》2023年第9期1-8,共8页China Medical Engineering

摘　　要：目的探讨α-1,6岩藻糖基转移酶(FUT8)在食管鳞状细胞癌(ESCC)放射抵抗中的作用。方法利用Oncomine和GEPIA数据库分析FUT8在食管癌中的表达水平,利用String数据库分析FUT8表达相关分子。采用慢病毒转染技术,在亲代ESCC细胞中分别过表达及敲低FUT8的表达。给予6 Gy剂量的X线照射后,克隆形成实验和流式细胞术分析FUT8对细胞放射敏感性的影响。结果Oncomine和GEPIA数据库显示,FUT8在ESCC组织中的表达明显高于正常组织。String数据库分析显示FUT6、MGAT2、MGAT4A、MGAT4B、MGAT4D、MGAT5、B4GALT1、B4GALT2、B4GALT3等基因与FUT8有明显的相互作用。细胞实验表明,FUT8过表达后的ESCC亲本细胞,经6 Gy照射,克隆形成能力显著增强,细胞凋亡比例减少,G2/M期细胞阻滞减弱,放射敏感性下降;而敲低FUT8表达后的ESCC亲本细胞,经6 Gy照射,克隆形成能力显著减弱,细胞凋亡比例增加,G2/M期细胞阻滞增强,放射敏感性增强。结论FUT8在ESCC组织中高表达,且体外实验证实FUT8对ESCC的放射抗性具有调控作用,有望成为克服ESCC放射抵抗的新靶点,具有重要的临床意义。【Objective】To investigate the role ofα-1,6 fucosyltransferase(FUT8)in the radioresistance of esophageal squamous cell carcinoma(ESCC).【Methods】Oncomine and GEPIA databases were used to analyze the expression level of FUT8 in esophageal carcinoma,and String database was used to analyze the expression related molecules of FUT8.Lentiviral transfection was used to overexpress and knock down FUT8 in parental ESCC cells.After 6 Gy X-ray irradiation,the effect of FUT8 on the radiosensitivity of cells was analyzed by colony formation assay and flow cytometry.【Results】Oncomine and GEPIA database showed that the expression of FUT8 in ESCC was significantly higher than that in normal tissues.String database analysis showed that FUT6,MGAT2,MGAT4A,MGAT4B,MGAT4D,MGAT5,B4GALT1,B4GALT2,B4GALT3 and other genes had obvious interaction with FUT8.Cell experiments showed that the colony forming ability of ESCC parental cells overexpressing FUT8 was significantly enhanced,the percentage of apoptosis was reduced,the cell arrest in G2/M phase was weakened,and the radiosensitivity was decreased after 6 Gy irradiation.After 6 Gy irradiation,the parental ESCC cells with FUT8 knock-down expression showed significantly decreased clonogenic ability,increased apoptosis rate,enhanced G2/M phase arrest and enhanced radiosensitivity.【Conclusion】FUT8 is highly expressed in ESCC,and it is proved that FUT8 can regulate the radioresistance of ESCC in vitro.FUT8 may become a new target to overcome the radioresistance of ESCC and has important clinical significance.

关 键 词：α-1 6岩藻糖基转移酶 食管鳞状细胞癌 糖基化 放疗抵抗 

分 类 号：R735.1[医药卫生—肿瘤]