C_(18)-亲水相互作用柱串联高效液相色谱法测定咖啡因  

Determination of Caffeine by High Performance Liquid Chromatography with C_(18)-Hydrophilic Interaction Liquid Chromatography Column Tandem

作　　者：司晓喜 王丹[2] 许志刚[2] 刘硕 刘志华 刘春波 李振杰 朱瑞芝 张凤梅 李世卫 Si Xiaoxi;Wang Dan;Xu Zhigang;Liu Shuo;Liu Zhihua;Liu Chunbo;Li Zhenjie;Zhu Ruizhi;Zhang Fengmei;Li Shiwei(Yunnan Key Laboratory of Tobacco Chemistry,Research and Development Center of China Tobacco Yunnan Industrial Company Limited,Kunming,Yunnan 650231,China;Faculty of Science,Kunming University of Science and Technology,Kunming,Yunnan 650500,China)

机构地区：[1]云南中烟工业有限责任公司技术中心、云南烟草化学重点实验室,云南昆明650231 [2]昆明理工大学理学院,云南昆明650500

出　　处：《化学世界》2023年第5期348-352,共5页Chemical World

基　　金：云南中烟工业有限责任公司烟草化学重点实验室(No.2021539200340247);云南省教育厅科学研究基金(No.2021Y114)资助项目。

摘　　要：建立了C_(18)-色谱柱串联亲水相互作用色谱(HILIC)柱高效测定咖啡饮料中咖啡因含量的方法。对色谱分离条件进行了优化,实验结果表明最佳的色谱分离条件为:甲醇/水=30∶70(V∶V),流速为0.5 mL/min,柱温为30℃,检测波长为272 nm。在4个咖啡饮料中均检出了咖啡因,咖啡饮料检出量为10.36~29.46 mg/L,相对标准偏差(RSD)不高于3%。检测下限(S/N=3)为0.1 mg/L。为验证方法的准确性,进一步开展了加标回收实验,4个咖啡饮料中咖啡因的回收率为81.08%~119.32%。该方法能满足实际样品中咖啡因的检测。并且双柱串联技术还消除了基质效应,该方法能准确、高效、灵敏地测定实际样品中的咖啡因。C_(18)-Hydrophilic interaction double-column tandem method was established to efficiently determine caffeine in coffee beverage.The chromatographic separation conditions were optimized and experimental results showed that the best conditions were mobile phase of methanol/water=30∶70(V∶V)with a flow rate of 0.5 mL/min,a column temperature of 30℃and a detection wavelength of 272 nm.Caffeine was detected in all four coffee beverages,the detected amount was 10.36~29.46 mg/L and the relative standard deviation(RSD)was below 3%.The lower detection limit(S/N=3)was 0.1 mg/L.In order to verify the accuracy of the method,further experiments were carried out to add standard recovery.The recoveries of 4 kind coffee beverages were between 81.08%and 119.32%.This method can satisfy the detection of caffeine in actual samples.In addition,the double-column tandem technology also eliminates the matrix effect so that this method can accurately,efficiently and sensitively determine the caffeine in actual samples.

关 键 词：柱串联 HILIC柱 C_(18)柱 咖啡因 

分 类 号：O652.63[理学—分析化学]