白花重楼叶绿体基因组特征及系统发育分析  

Characterization and Phylogenetic Analysis of the Complete Chloro-plast Genome of Paris polyphylla var.alba(Melanthiaceae)

作　　者：张书东 凌立贞 谢丹丹 刘祯珍 ZHANG Shudong;LING Lizhen;XIE Dandan;LIU Zhenzhen(Liupanshui Normal University/Key Laboratory of Liupanshui City for Study and Utilization of Ethnic Medicinal Plant Resources of Western Guizhou Province,Liupanshui,Guizhou 553004,China;College of Life and Health,Dalian University,Dalian,Liaon-ing 116622,China)

机构地区：[1]六盘水师范学院/贵州西部民族药用植物资源研究与利用重点实验室,贵州六盘水553004 [2]大连大学生命健康学院,辽宁大连116622

出　　处：《热带作物学报》2023年第8期1551-1560,共10页Chinese Journal of Tropical Crops

基　　金：贵州省普通高等学校科技拔尖人才项目(黔教合KY字[2019]061);六盘水市科技计划项目(No.52020-2019-05-05,No.52020-2022-PT-20)。

摘　　要：白花重楼(Paris polyphylla var. alba)为藜芦科(Melanthiaceae)重楼属药用植物,为国家二级重点保护植物。随着重楼原料药需求量逐渐上涨,白花重楼野生资源遭到严重破坏。为保护白花重楼遗传资源和探讨该物种的系统位置,本研究对白花重楼的全基因组进行高通量测序,通过组装和注释获得其完整的叶绿体基因组序列。对其基因组结构特性和系统发育关系分析结果表明,白花重楼叶绿体基因组全长163 944 bp,大单拷贝区(large single-copy, LSC)、小单拷贝区(small single-copy region, SSC)和反向互补重复区(inverted repeats, IR)长度分别为84 179、12 967、33 399 bp,为典型的四分体结构。白花重楼叶绿体基因组共编码134个基因,包括88个蛋白质编码基因,38个tRNA基因和8个rRNA基因。白花重楼叶绿体基因组共检测到645个长重复序列和97个SSR(simple sequence repeat)位点,长重复序列以正向重复和回文重复为主,SSR以单碱基重复A/T类型为主。基于重楼属34种(含种下单位)叶绿体全基因组构建的系统进化树显示,重楼属为单系类群,可划分为5个大的分支,七叶一枝花各变种虽然均位于蚤休组,但并不构成单系,白花重楼与毛重楼亲缘关系最近。本研究通过二代测序数据组装获得了完整的白花重楼叶绿体基因组序列,明确了白花重楼在重楼属中的系统位置,为研究重楼属植物的系统进化、物种鉴定和资源保护提供科学依据。Paris polyphylla var.alba,a medicinal plant of Melanthiaceae,is classed as the national second-grade pro-tected species of China.With the increasing demand for raw materials of Rhizoma paridis,the wild resources of P.polyphylla var.alba have been seriously damaged.In order to protect the genetic resources and clarify the systematic positon of this species,Illumina Hiseq X Ten platform was used to sequence the chloroplast(cp)genome.The structural characteristics of the complete cp genome sequence and phylogenetic relationship of P.polyphylla var.alba in Paris were analyzed.The results showed that the cp genome was 163944 bp in length with a typical quadripartite structure with a large single-copy(LSC)region of 84179 bp,a small single-copy(SSC)region of 12967 bp,and a pair of 33399 bp inverted repeats(IRs).The genome contained 134 genes,including 88 protein-coding genes(PCGs),38 tRNA genes and 8 rRNA genes.A total of 645 long repeats and 97 simple sequence repeats(SSRs)loci were detected in the complete cp genome,in which forward and palindromic repeats were the mainly long repeat types,and most of SSRs had A/T base preference.Phylogenetic analysis based on the complete cp genomes showed that Paris formed a monophyletic group,and could be further divided into five major clades corresponding to five sections,respectively.The varieties of P.polyphylla are located in Sect.Euthyra,but are not clustered a monophyletic group,and P.polyphylla var.alba is closely related to P.mairei.This study would provide a scientific basis for studying the phylogeny,species identification and resource conservation of Paris.

关 键 词：白花重楼 重楼属 藜芦科 系统发育 叶绿体基因组 

分 类 号：S567.239[农业科学—中草药栽培]