Are globoseries glycosphingolipids SSEA-3 and-4 markers for stem cells derived from human umbilical cord blood?  

作　　者：Heli Suila Virve Pitka¨nen Tia Hirvonen Annamari Heiskanen Heidi Anderson Anita Laitinen Suvi Natunen Halina Miller-Podraza Tero Satomaa Jari Natunen Saara Laitinen Leena Valmu 

机构地区：[1]Research and Development,Finnish Red Cross Blood Service,R&D,Kivihaantie 7,FIN-00310 Helsinki,Finland [2]Glykos Finland Ltd,Viikinkaari 6,00790 Helsinki,Finland [3]Department of Medical Chemistry and Cell Biology,Institute of Biomedicine,Gothenburg Universit,P.O.Box 440,SE 40530 Gothenburg,Sweden

出　　处：《Journal of Molecular Cell Biology》2011年第2期99-107,共9页分子细胞生物学报（英文版）

基　　金：This work was supported by the Finnish Funding Agency for Technology and Innovation(TEKES);the EVO Medical Research Fund of Finnish Red Cross Blood Service and The Finnish Glycoscience Graduate School.

摘　　要：Umbilical cord blood(UCB)is an efficient and valuable source of hematopoietic stem cells(HSCs)for transplantation.In addition to HSCs it harbours low amounts of mesenchymal stem cells(MSCs).No single marker to identify cord blood-derived stem cells,or to indicate their multipotent phenotype,has been characterized so far.SSEA-3 and-4 are cell surface globoseries glycosphingolipid epitopes that are commonly used as markers for human embryonic stem cells,where SSEA-3 rapidly disappears when the cells start to differentiate.Lately SSEA-3 and-4 have also been observed in MSCs.As there is an ongoing discussion and variation of stem-cell markers between laboratories,we have now comprehensively characterized the expression of these epitopes in both the multipotent stem-cell types derived from UCB.We have performed complementary analysis using gene expression analysis,mass spectrometry and immunochemical methods,including both flow cytometry and immunofluoresence microscopy.SSEA-4,but not SSEA-3,was expressed on MSCs but absent from HSCs.Our findings indicate that SSEA-3 and/or-4 may not be optimal markers for multipotency in the case of stem cells derived from cord blood,as their expression may be altered by cell-culture conditions.

关 键 词：umbilical cord blood hematopoietic stem cells mesenchymal stem cells SSEA-3 SSEA-4 

分 类 号：R73[医药卫生—肿瘤]