机构地区:[1]福建农林大学林学院,福州350002 [2]森林生态系统过程与经营福建省高校重点实验室,福州350002 [3]福建省惠安赤湖国有防护林场,福建惠安362100
出 处:《植物生态学报》2023年第6期804-821,共18页Chinese Journal of Plant Ecology
基 金:国家自然科学基金(32071753)。
摘 要:为明晰内生真菌在宿主植物耐盐生长中是否存在拮抗作用,探讨利用内生真菌提高困难立地条件下造林成活率的可能,将两株耐盐内生真菌葡萄座腔菌(Botryosphaeria sp.)Z1(T3)、炭团菌(Hypoxylon sp.)Y6(T4)及其混合菌(T2)进行固体发酵培养,添加到栽植有木麻黄(Casuarina equisetifolia)幼苗的不同盐度(质量分数为0、3‰、6‰、9‰)盆钵土壤中,以添加菌株载体(T1)和无添加(CK)为对照,研究固体培养内生真菌对土壤盐胁迫下木麻黄幼苗抗逆生理特征的影响及其与生物量的关系。结果表明:菌株处理显著提高了盐胁迫下幼苗的生物量,幼苗生理特征受土壤盐度、时间和菌株种类影响较大。3‰盐度下,T2处理15天后可溶性糖(SS)含量、可溶性蛋白(SP)含量、超氧化物歧化酶(SOD)活性以及45天后过氧化氢酶(CAT)活性高于CK、T1,相对电导率(REC)在T2、T3和T4处理胁迫期都显著降低;9‰盐度下,T2、T3和T4处理实验期内REC、H2O2含量显著降低,胁迫60天后丙二醛(MDA)含量显著降低,SS含量、SOD活性、CAT活性显著增加,T2处理的SP含量、过氧化物酶(POD)活性同样显著增加。采用逐步回归建立了菌株处理下幼苗生理特征与生物量关系的回归方程模型,通径分析表明REC、POD活性和SS含量为影响生物量的主要生理因素。综上,不同菌株对幼苗渗透调节物质含量、氧化酶活性和生物量的影响不同,混合菌的拮抗作用最为明显,研究结果初步揭示了盐胁迫下木麻黄渗透调节物质含量、抗逆酶、生物量与木麻黄内生真菌的关系,为木麻黄抗逆工程菌的发掘提供了研究基础。Aims Endophyte is a new microbial resource,which has a complex micro-ecological relationship with plants.In this paper,the antagonistic effect of endophytic fungi on the growth of salt-tolerant plants was confirmed by adding endophytic fungi to the plants and testing the physiological characteristics and biomass of the seedlings.Methods Two salt-tolerant endophytic fungi Botryosphaeria sp.Z1(T3),Hypoxylon sp.Y6(T4),and their mixture(T2)were cultured in solid fermentation and added to potted soil planted with seedlings of Casuarina equisetifolia at different salinity(mass fraction:0,3‰,6‰and 9‰),which were treated with an added fungi of seedlings.The addition of microbiological medium(T1)and no added microbiological medium(CK)were treated as two experimental controls,which was to confirm the effect of the fungi,but not the microbiological medium.Samples were taken at 15 day intervals,four times,for a total of 60 days,and the relative electronic conductivity(REC),malondialdehyde(MDA)content,soluble sugar(SS)content,soluble protein(SP)content,proline(PRO)content,superoxide dismutase(SOD)activity,peroxidase(POD)activity,catalase(CAT)activity,reactive oxygen(H2O2)content of leaves and the biomass of seedling were determined each time.Important findings We found that the biomass of seedlings treated by fungal strain was significantly increased under salt stress,and the physiological characteristics were significantly affected by soil salinity,time and fungal species.At 3‰salinity,the contents of SS and SP,and the activity of SOD and CAT were higher in T2 treatment than in CK and T1 after 15 days of treatment,but the REC was significantly lower in T2,T3 and T4 treatments during the stress period.At 9‰salinity,T2 treatment significantly reduced the REC and the contents of H2O2 and MDA,but significantly increased the content of SS and the activity of SOD and CAT.Especially,SP content and POD activity were also significantly increased in T2 treatment after 60 days.A stepwise regression was used to model the relat
关 键 词:木麻黄 土壤盐度 内生真菌 植物生理 生物量 通径分析
分 类 号:S792.99[农业科学—林木遗传育种]
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