c-Fos、Cyclin-D1、CDK4在乳腺癌中的表达及相关性分析  

Expression and correlation analysis of c-Fos,Cyclin-D1 and CDK4 in breast cancer

作　　者：胡文慧 居红格[1,2] 李峰[2] 张伟[1,2] 马俊兵 陈鹏[2] 李园园 孙杨 HU Wenhui;JU Hongge;LI Feng;ZHANG Wei;MA Junbing;CHEN Peng;LI Yuanyuan;SUN Yang(Baotou Medical College,Baotou 014040,China;Department of Pathology,First Affiliated Hospital of Baotou Medical College)

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]内蒙古科技大学包头医学院第一附属医院病理科

出　　处：《包头医学院学报》2023年第9期1-7,共7页Journal of Baotou Medical College

基　　金：包头市科技计划项目(2020Z1016-2);包头医学院科学研究基金项目(BYJJ-ZRQM202205)。

摘　　要：目的:探讨癌基因c-Fos、细胞周期蛋白D1(cyclin-D1)、细胞周期蛋白依赖性激酶4(CDK4)在乳腺癌及癌旁组织中的表达情况以及其与临床病理特征相关性,并进行生存分析。方法:随机选取20例乳腺癌及癌旁组织进行蛋白印迹实验(western blot,WB)和实时定量反转录聚合酶链反应(real-time quantitative reverse transcription-polymerase chain reaction,RT-qPCR),分别检测乳腺癌组织中c-Fos的蛋白表达和c-Fos、Cyclin-D1、CDK4的mRNA含量;购买乳腺癌组织芯片进行免疫组织化学(immunohistochemistry,IHC)实验,研究c-Fos、Cyclin-D1、CDK4的表达与乳腺癌临床病理特征及预后的关系。结果:WB实验结果提示c-Fos在乳腺癌组织表达高于癌旁组织,差异有统计学意义(P<0.05);RT-qPCR实验结果提示乳腺癌组织中c-Fos mRNA含量与癌旁组织比较差异无统计学意义(P>0.05),乳腺癌组织中Cyclin-D1 mRNA、CDK4 mRNA含量高于癌旁组织,差异有统计学意义(P<0.05);IHC实验表明c-Fos、Cyclin-D1、CDK4在乳腺癌组织中的表达明显高于癌旁组织,差异有统计学意义(P<0.05);癌组织中三者的表达均呈正相关(r=0.320,r=0.486,r=0.514,P<0.05);在乳腺癌组织中,c-Fos的表达与临床分期有关(P<0.05);Cyclin-D1的表达与脉管侵犯有关(P<0.05);CDK4的表达与淋巴结转移有关(P<0.05);生存分析提示三者的高表达是乳腺癌患者总生存时间的危险因素。结论:乳腺癌组织中c-Fos、Cyclin-D1、CDK4高表达并存在正相关性,且与乳腺癌的临床病理特征相关,提示共同参与乳腺癌的发生及发展过程;三者的高表达影响乳腺癌患者的预后。Objective:To observe the expression of oncogene c-Fos,cyclin D1 and cyclin dependent kinase 4(CDK4)in breast cancer and para-cancerous tissues and their correlation with clinicopathological features,and to analyze the survival of breast cancer.Methods:Western blot(WB)and real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR)were used to detect the expression level of c-Fos protein and mRNA content of c-Fos,Cyclin-D1 and CDK4 in 20 cases of breast cancer and para-cancerous tissues.Breast tumor-on-a-chip was purchased to do immunohistochemical(IHC)experiment to analyze the relationship between expression levels of c-Fos,Cyclin-D1 and CDK4 and the clinicopathological features and prognosis of breast cancer.Results:WB test showed that the expression level of c-Fos protein in breast cancer was significantly higher than that in para-cancerous tissues,and the difference was statistically significant(P<0.05).RT-qPCR method showed that the mRNA content of c-Fos in breast cancer was higher than that in para-cancerous tissues,however,the difference was not statistically significant(P>0.05).The mRNA contents of Cyclin-D1 and CDK4 in breast cancer were significantly higher than those in para-cancerous tissues(P<0.05).IHC experiment showed that the expression levels of c-Fos,Cyclin-D1 and CDK4 in breast cancer tissues were significantly higher than those in para-cancerous tissues,and the differences were statistically significant(P<0.05).The expression levels of the three proteins in cancer tissues were all positively correlated(r=0.320,r=0.486,r=0.514,P<0.05).In breast cancer,the expression level of c-Fos was correlated to clinical stage(P<0.05),the expression level of Cyclin-D1 was correlated to vascular invasion(P<0.05),the expression level of CDK4 was related to lymph node metastasis(P<0.05),and the high expression of the three proteins was the risk factor to the over survival of breast cancer patients.Conclusion:c-Fos,Cyclin-D1 and CDK4 are highly expressed in breast cancer,and there is a positive

关 键 词：C-FOS CYCLIN-D1 CDK4 乳腺癌 免疫组化 生存分析 

分 类 号：R737.9[医药卫生—肿瘤]