皮质发育不良相关药物难治性癫痫患者的血浆微小RNA表达谱  

Plasma micro RNA expression profiles in patients with drug-refractory epilepsy associated with focal cortical dysplasia

作　　者：孙宇强 李庆伟[1] 张远洋 闫晓枫[2] 荣维娜[1] 宋新宇 程化坤[1] Sun Yuqiang;Li Qingwei;Zhang Yuanyang;Yan Xiaofeng;Rong Weina;Song Xinyu;Cheng Huakun(Department of Neurosurgery,Heilongjiang Provincial Hospital,Haerbin 150036,Heilongjiang,China;Department of Clinical Laboratory,Heilongjiang Provincial Hospital,Haerbin 150036,Heilongjiang,China)

机构地区：[1]黑龙江省医院神经外科,黑龙江哈尔滨150036 [2]黑龙江省医院检验科,黑龙江哈尔滨150036

出　　处：《实用检验医师杂志》2023年第2期204-208,共5页Chinese Journal of Clinical Pathologist

基　　金：黑龙江省卫生健康委科研课题(20210404040146);黑龙江省自然科学基金(LH2020H118)。

摘　　要：目的研究皮质发育不良(FCD)相关药物难治性癫痫患者的血浆微小RNA(miRNA)表达谱。方法从辽宁省癫痫疾病诊疗中心术后切除的大脑致痫灶皮质标本中挑选出9份经病理证实符合FCD的样本,同时选取8份正常脑组织样本作为对照皮质。另外选择同期在黑龙江省医院手术治疗的60例FCD相关药物难治性癫痫患者作为研究对象,并根据不同病程、发作频率、预后分组;分别采集手术切除的患者致痫灶脑皮质组织及血浆作为检测标本。比较FCD和正常脑组织中以及FCD相关难治性癫痫患者脑组织和血浆中miRNA-129-2-3p、miRNA-4521、miRNA-935、miRNA-323a-5p及miRNA-485-3p的相对表达量;比较不同病程、发作频率、预后患者血浆中miRNA-129-2-3p、miRNA-4521、miRNA-935、miRNA-323a-5p及miRNA-485-3p的表达量。绘制受试者工作特征曲线(ROC曲线)并计算ROC曲线下面积(AUC),分析血浆中miRNA对FCD相关药物难治性癫痫的诊断价值。结果FCD脑组织中miRNA-129-2-3p、miRNA-4521、miRNA-935、miRNA-323a-5p及miRNA-485-3p的相对表达量较对照组明显更高(miRNA-129-2-3p:6.36±1.24比2.06±0.38,miRNA-4521:2.04±0.23比1.11±0.36,miRNA-935:2.12±0.25比1.14±0.37,miRNA-323a-5p:3.48±0.49比1.06±0.24,miRNA-485-3p:3.26±0.29比1.02±0.17,均P<0.05)。FCD相关难治性癫痫患者血浆中miRNA-129-2-3p的相对表达量较脑组织明显更高(5.78±1.36比4.33±1.27,P<0.05)。病程≥5年、发作频率≥4次以及预后不良患者血浆中miRNA-129-2-3p表达量分别较病程<5年、发作频率<4次以及预后良好患者明显升高。ROC曲线分析显示,血浆中miRNA-129-2-3p对FCD相关药物难治性癫痫的诊断价值最高,AUC为0.894,敏感度为93.66%。结论FCD组织中miRNA-129-2-3p、miRNA-4521、miRNA-935、miRNA-323a-5p及miRNA-485-3p均呈高表达,血浆中的miRNA-129-2-3p高表达可能对FCD相关难治性癫痫患者具有较好的诊断效果,值得临床关注。Objective To study the plasma micro RNA(miRNA)expression profile in patients with focal cortical dysplasia(FCD)-related drug-refractory epilepsy.Methods From the cortical specimens of cerebral epileptogenic lesions removed after surgery at Liaoning Epilepsy Disease Diagnosis and Treatment Center,9 samples with pathologically confirmed FCD were selected,and 8 normal brain samples were selected as control cortex.In addition,60 patients with FCD related drug-refractory epilepsy treated in Heilongjiang Provincial Hospital during the same period were selected as study objects and grouped according to different disease duration,seizure frequency and prognosis.Cerebral cortical tissue and plasma of patients were collected as test specimens.The relative expression of miRNA-129-2-3p,miRNA-4521,miRNA-935,miRNA-323a-5p and miRNA-485-3p in FCD and normal brain tissue,as well as in brain tissue and plasma of FCD related refractory epilepsy patients were compared.The expression of miRNA-129-2-3p,miRNA-4521,miRNA-935,miRNA-323a-5p and miRNA-485-3p in the plasma of patients with different disease courses,seizure frequencies and prognosis was compared.The receiver operator characteristic(ROC)curve was drawn and the area under ROC curve(AUC)was calculated to analyze the diagnostic value of miRNA in plasma for drug-refractory epilepsy related to FCD.Results The relative expression of miRNA-129-2-3p,miRNA-4521,miRNA-935,miRNA-323a-5p and miRNA-485-3p in FCD tissue was higher than that in control group(miRNA-129-2-3p:6.36±1.24 vs.2.06±0.38,miRNA-4521:2.04±0.23 vs.1.11±0.36,miRNA-935:2.12±0.25 vs.1.14±0.37,miRNA-323a-5p:3.48±0.49 vs.1.06±0.24,miRNA-485-3p:3.26±0.29 vs.1.02±0.17,all P<0.05).The relative expression of miRNA-129-2-3p in plasma of FCD-related refractory epilepsy patients was higher than that in brain tissue(5.78±1.36 vs.4.33±1.27,P<0.05).The plasma expression of miRNA-129-2-3p in patients with desease course≥5 years,frequency≥4 and poor prognosis was higher than those with desease course<5 years,frequency<

关 键 词：皮质发育不良 难治性癫痫 血浆 微小RNA表达谱 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R446.1[医药卫生—临床医学]