Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway  

作　　者：Yijun Chen Zhujin Lu Jiaxin Feng Zefeng Chen Zejian Liu Xiuqi Wang Huichao Yan Chunqi Gao 

机构地区：[1]College of Animal Science,South China Agricultural University/Guangdong Provincial Key Laboratory of Animal Nutrition Control/State Key Laboratory of Swine and Poultry Breeding Industry,Guangzhou 510642,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第8期1213-1221,共9页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(No.31972585);the Special Fund Support Project for Scientific and Technological Innovation Cultivation of Guangdong University Students(No.PDJH2022B0084);the Technical System of Poultry Industry of Guangdong Province,China(No.2023KJ128).

摘　　要：Roof plate-specific spondin 1(R-spondin1,RSPO1)is a Wnt/β-catenin signaling pathway activator that binds with Wnt ligands to stimulate the Wnt/β-catenin signaling pathway,which is key to hair regeneration.However,it is not clear whether recombinant RSPO1(rRSPO1)affects hair regeneration.Here,we treat C57BL/6 male mice with rRSPO1 and investigate the expression of the Wnt/β-catenin signaling pathway and the activation of hair follicle stem cells in the dorsal skin.The mouse skin color score and hair-covered area are determined to describe hair growth,and the skin samples are subjected to H&E staining,western blot analysis and immunofluorescence staining to evaluate hair follicle development and the expressions of Wnt/β-catenin signaling pathway-related proteins.We find that rRSPO1 activates mouse hair follicle stem cells(mHFSCs)and accelerates hair regeneration.rRSPO1 increases the hair-covered area,the number of hair follicles,and the hair follicle diameter and length.Moreover,rRSPO1 enhances the activity of Wnt/β-catenin signaling pathway-related proteins and the expressions of HFSC markers,as well as mHFSC viability.These results indicate that subcutaneous injection of rRSPO1 can improve hair follicle development by activating the Wnt/β-catenin signaling pathway,thereby promoting hair regeneration.This study demonstrates that rRSPO1 has the potential to treat hair loss by activating the Wnt/β-catenin signaling pathway.

关 键 词：roof plate-specific spondin 1(RSPO1) hair follicle stem cells hair regeneration Wnt/β-catenin signaling pathway MICE 

分 类 号：R730.5[医药卫生—肿瘤]