非小细胞肺癌病人血长链非编码RNA和微小RNA-191表达水平及临床意义  

Expression levels and clinical significance of plasma lncRNA ANRIL and miR-191 in patients with non-small cell lung cancer

作　　者：艾力江·多力坤 艾孜子·阿不来提 陈康[1] Ailijiang·Duolikun;Aizizi·Abulaiti;CHEN Kang(Department of Thoracic Surgery,Xinjiang Uygur Autonomous Region People's Hospital,Xinjiang,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院胸外科,乌鲁木齐830001

出　　处：《临床外科杂志》2023年第8期729-732,共4页Journal of Clinical Surgery

摘　　要：目的 观察长链非编码RNA(lncRNA,ANRIL)、微小RNA(miR)-191在非小细胞肺癌(NSCLC)病人血浆中的表达及与临床意义。方法 2015年5月~2017年8月我院首次确诊非小细胞肺癌(NSCLC)病人153例,健康体检者75例。采用实时荧光定量PCR(qRT-PCR)检测血浆ANRIL、miR-191水平,分析ANRIL、miR-191表达水平与临床病理特征的关系。Kaplan-Meier分析ANRIL、miR-191表达水平与预后的关系,Cox回归分析影响NSCLC病人预后不良的危险因素。结果 NSCLC病人血浆ANRIL水平为(3.09±0.53)、miR-191水平为(2.82±0.47),正常人群分别为(0.64±0.04)、(0.42±0.07),差异有统计学意义(P<0.05)。中低分化、Ⅲ+Ⅳ分期、有淋巴结转移病人血浆ANRIL、miR-191含量高于高分化、Ⅰ+Ⅱ分期、无淋巴结转移病人,差异有统计学意义(P<0.05)。ANRIL高表达组病人5年总体生存率(OS)为44.58%,ANRIL低表达组为73.85%;miR-191高表达组病人5年OS为50.63%,miR-191低表达组为71.01%,差异有统计学意义(P<0.05)。Ⅲ+Ⅳ期、有淋巴结转移、血浆ANRIL高表达及血浆miR-191高表达是影响NSCLC病人预后不良的独立危险因素(均P<0.05)。结论 NSCLC病人血浆ANRIL、miR-191高表达,与分期、淋巴结转移、分化程度及预后相关。Objective To observe the expression and and clinical significance of long non-coding RNA(lncRNA) ANRIL and miR-191 in the plasma of patients with non-small cell lung cancer(NSCLC).Methods 153 patients with NSCLC first diagnosed in our hospital from May 2015 to August 2017 and 75 healthy physical examiners were selected.Plasma ANRIL and miR-191 levels were detected by quantitative real-time fluorescence PCR(qRT-PCR).The relationship between ANRIL and miR-191 expression levels and clinicopathological characteristics was analyzed.Kaplan-Meier was used to analyze the relationship between ANRIL and miR-191 expression levels and prognosis.COX regression was used to analyze the risk factors for poor prognosis of NSCLC patients.Results Plasma ANRIL(3.09±0.53) and miR-191(2.82±0.47) levels were higher in NSCLC patients than in the normal population(0.64±0.04) and(0.42±0.07)(P<0.05).Plasma ANRIL and miR-191 levels were higher in patients with low to moderate differentiation,stage Ⅲ+Ⅳ,and lymph node metastasis than in patients with high differentiation,stage Ⅰ+Ⅱ,and no lymph node metastasis(all P<0.05).5-year overall survival(OS) was lower in patients in the high ANRIL expression group(37/83,44.58%),the miR-191 high expression group(40/79,50.63%)than in the low ANRIL expression group(48/65,73.85%),the miR-191 low expression group(49/69,71.01%)(both P<0.05).Stage Ⅲ+Ⅳ,presence of lymph node metastasis,high plasma ANRIL expression and high plasma miR-191 expression were independent risk factors for poor prognosis in NSCLC patients(all P<0.05).Conclusion High plasma ANRIL and miR-191 expression in NSCLC patients were associated with stage,lymph node metastasis,differentiation degree and prognosis.

关 键 词：非小细胞肺癌 微小RNA-191 长链非编码RNA 预后 

分 类 号：R734.2[医药卫生—肿瘤]