激光诱导大鼠CNV形态变异性的体内和体外特性研究  

Laser-induced Morphological Variability of Choroidal NeovasculaRization in Rats:in vivo and in vitro Characterization Studies

作　　者：曹明芳[1] 石楠楠 李婧[1] 袁智 王大池 姜洲洲 CAO Mingfang;SHI Nannan;LI Jing;YUAN Zhi;WANG Dachi;JIANG Zhouzhou(People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China;First Clinical Medical College,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学附属人民医院,福州350004 [2]福建中医药大学第一临床医学院,福州350122

出　　处：《激光生物学报》2023年第4期321-329,344,共10页Acta Laser Biology Sinica

基　　金：福建省自然科学基金项目(2020J011046);福建中医药大学附属人民医院中药制剂研发项目(ZYZJ2019003)。

摘　　要：532 nm激光光凝是诱导啮齿类动物脉络膜新生血管(CNV)的一种既定方法。本研究旨在评价激光诱导CNV在体内和体外随时间变化的形态变异程度,为今后CNV相关研究提供观察指标。本实验首先对棕色挪威(BN)大鼠进行激光造模,随后,根据不同时间点对其进行体内眼底照相、光学相干断层扫描(OCT)、眼底荧光血管造影(FFA)和吲哚菁绿血管造影(ICGA),最后,应用组织病理检查进行体外验证。结果发现,光凝后第7天出现CNV,第21天的CNV总发生率最高。FFA呈典型圆盘状强荧光渗漏,ICGA显示光凝区周边强荧光,中央低荧光,呈花环状形态。光学显微镜下可见,造模后第7天,视网膜下CNV形成,第7~21天,CNV中央厚度逐渐增加,第28天,CNV中央厚度保持相对稳定。这提示,532 nm激光诱导BN大鼠CNV模型造模时间短,成功率高。这种造模方式可以广泛应用于临床前实验研究,为进一步研究CNV发病机制提供了思路。532 nm laser photocoagulation is an established method to induce choroidal neovascularization(CNV)in rodents.532 nm laser photocoagulation is an established method for inducing CNV in rodents.The aim of this study was to evaluate the degree of morphological variation of laser-induced CNV in vivo and in vitro over time so as to provide an observational metric for future CNV-related experiments.In this experiment,brown Norway rats were first laser-molded.Subsequently,in vivo fundus photography,optical coherence tomography(OCT),fundus fluorescence angiography(FFA)and indocyanine green angiography(ICGA)were performed according to different time points,and finally histopathological examination was applied for in vitro validation.The results showed that CNV appeared at 7th day after photocoagulation,with the highest total incidence of CNV at 21th day.FFA showed typical disc-like strong fluorescence leakage,while ICGA displayed strong fluorescence at the periphery of the photocoagulation area and low fluorescence in the center with a wreath-like morphology.Subretinal CNV were formed 7th day after molding as seen under optical microscope.The central thickness of the CNV gradually increased from 7th to 21th day and remained relatively stable until 28th day.This suggests that the modeling approach utilizing 532 nm laser induced BN rats with CNV exhibits a short duration and high success rate,rendering it applicable for preclinical experimental studies and providing insights for further research on the pathogenesis of CNV.

关 键 词：激光诱导 532 nm 脉络膜新生血管 动物模型 BN大鼠 

分 类 号：R774[医药卫生—眼科] Q95-3[医药卫生—临床医学]