生物信息辅助斑马鱼模型揭示EHDPP诱发代谢疾病的机制  

Bioinformatics-assisted zebrafish model reveals the mechanism of EHDPP-induced metabolic disease

作　　者：倪安煜 李金芸 方露 万可维 陈泱 王慧利 闫瑾 NI An-yu;LI Jin-yun;FANG Lu;WAN Ke-wei;CHEN Yang;WANG Hui-li;YAN Jin(The school of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009

出　　处：《中国环境科学》2023年第9期4942-4950,共9页China Environmental Science

基　　金：国家自然科学基金资助项目(22106118)。

摘　　要：通过对CTD和GEO等公共数据库中EHDPP信息的挖掘,发现EHDPP通过调控一系列细胞膜受体介导的生物信号传导过程,促进代谢紊乱和炎症的发展,有诱发相关代谢性疾病的风险.为此,本研究以斑马鱼幼鱼为模型暴露于亚致死剂量的EHDPP中,进一步对该结果进行验证.结果显示,暴露于500μg/L的EHDPP 5天后斑马鱼幼鱼体内的甘油三酯和总胆固醇含量分别上升了53.8%和32.1%.此外,qRT-PCR结果表明Pparγ介导的脂质代谢途径可能是EHDPP在斑马鱼中引起的异常脂质积累的潜在关键机制.This study utilized data mining from public databases,such as CTD and GEO,to demonstrate that EHDPP has the risk of inducing metabolic diseases by modulating various cell membrane receptor-mediated biological signaling pathways,which are related to the development of metabolic disorders and inflammation.Then,zebrafish larvae were utilized as models and exposed to a sublethal dose of EHDPP in order to validate the aforementioned findings.The findings revealed that after 5days of 500μg/L-EHDPP exposure,the content of triglycerides and total cholesterol in zebrafish larvae increased by 53.8%and 32.1%,respectively.Additionally,qRT-PCR results indicated that the lipid metabolism pathway mediated by Pparγmay serve as a potential pivotal mechanism for the induction of abnormal lipid accumulation in zebrafish caused by EHDPP.

关 键 词：2-乙基己基二苯基磷酸酯 肥胖 代谢疾病 生物信息学分析 斑马鱼 

分 类 号：X174[环境科学与工程—环境科学]