基于基因表达谱数据筛选膀胱尿路上皮癌潜在的治疗药物  

Screening potential therapeutic drugs for bladder urothelial carcinoma based on gene expression profile data

作　　者：管波 单卫民 李明 GUAN Bo;SHAN Weiming;LI Ming(Department of Urology,Fuyang People’s Hospital,Fuyang 236012,China)

机构地区：[1]安徽省阜阳市人民医院泌尿外科,236012

出　　处：《现代泌尿生殖肿瘤杂志》2023年第4期203-207,共5页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：2020年阜阳市自筹经费科技计划项目(FK202081018)。

摘　　要：目的基于癌症基因组图谱(TCGA)及药物基因组学(CMap)数据库筛选膀胱尿路上皮癌(BLCA)潜在的治疗药物,为BLCA的药物治疗提供新的思路。方法提取TCGA数据库中BLCA患者和BLCA转录组的临床数据,通过WGCNA分析,选择与BLCA肿瘤分期、分级、生存状态、生存时间等临床特征相关的基因模块,并联合CMap数据库筛选BLCA患者候选的治疗药物。结果BLCA癌组织与正常膀胱组织中差异表达的RNA有3729种,通过WGCNA分析,BLCA转录组数据聚类为9个共表达的基因模块,选择与BLCA肿瘤分期、分级、生存状态等临床性状相关的基因模块,联合CMap数据库筛选出11种与该基因模块表达负相关的小分子药物。结论通过对TCGA数据库联合CMap数据库筛选出11种小分子药物进行分析,结果表明这些小分子药物可能对BLCA有潜在的治疗价值。Objective To screen potential therapeutic drugs for bladder urothelial carcinoma(BLCA)based on TCGA and CMAP database,and to provide a new idea for the drug treatment of BLCA.Methods Firstly,the clinical data and transcriptome data of BLCA patients in TCGA database were extracted.Through WGCNA analysis,the genes in the gene module related to the clinical characteristics of BLCA tumor stage,grade,survival status and survival time were selected.The selected gene module combined with CMap data was used to screen the candidate therapeutic drugs for BLCA patients.Results Three thousand seven hundred and twenty-nine kinds of RNA were differentially expressed in the normal bladder and BLCA tissues of 414 patients.Through WGCNA analysis,the transcriptome data of BLCA were aggregated into 9 co-expressed gene modules,and the genes in the gene modules related to the clinical traits such as BLCA tumor stage,grade and survival status were selected.Combined with CMap data,11 small molecule drugs negatively related to the expression of the gene module were screened.Conclusions Eleven small molecule drugs screened by TCGA database combined with CMap database may have potential value in the treatment of BLCA.

关 键 词：膀胱尿路上皮癌 TCGA数据库 CMap数据库 药物治疗 

分 类 号：R737.14[医药卫生—肿瘤]