高表面增强拉曼散射活性rGO-AuNPs的合成及其在氧氟沙星检测中的应用  

Synthesis of reduced graphene oxide-gold composite nanomaterials with high SERS activity and application of ofloxacin detection

作　　者：程欣蕾 杨武英[1] 杜娟[1] CHENG Xinlei;YANG Wuying;DU Juan(School of Food Science and Engineering,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China)

机构地区：[1]江西农业大学食品科学与工程学院,江西南昌330045

出　　处：《食品与机械》2023年第8期48-54,共7页Food and Machinery

基　　金：国家自然科学基金(编号:31901774);江西省现代农业产业技术体系(编号:JXARS-03)。

摘　　要：目的:为氧氟沙星含量检测提供新的研究方向。方法:利用生物质还原法合成具有高表面增强拉曼散射(SERS)活性的还原氧化石墨烯—金复合纳米材料(rGO-AuNPs),通过紫外可见光谱、透射电子显微镜、扫描电子显微镜证明rGO-AuNPs的成功合成,测量并计算其拉曼增强因子(EF)。基于SERS热点效应与核酸适配体的特异性结合能力建立OFL含量检测体系,优化检测条件,建立标准曲线,并将其应用于池塘水样OFL检测中。结果:以百合花瓣生物质成功合成了rGO-AuNPs,金纳米粒子生长在还原氧化石墨烯片层上,EF为3.88×10^(7)。体系SERS强度与氧氟沙星质量浓度在1~500 ng/mL范围内有较好的线性关系,检测限为0.3 ng/mL。加标回收率为96.28%~102.84%,相对标准偏差<10%。结论:合成的rGO-AuNPs具有高SERS活性,在OFL检测方面具有较好的应用前景。Objective:This study provided a new research direction for the detection of ofloxacin content.Methods:Using biomass reduction method to formulate a reduced graphene oxide-gold composite nanomaterials(rGO-AuNPs),which had high surface enhanced Raman scattering(SERS)activity.The successful synthesis of rGO-AuNPs was demonstrated by ultraviolet visible spectroscopy,transmission electron microscopy and scanning electron microscopy,respectively.The Raman enhancement factor(EF)of rGO-AuNPs was measured and calculated.Based on the SERS hot spot effect and the specific binding ability of nucleic acid aptamer,ofloxacin content detection system was established,the detection conditions were optimized,the standard curve was established,and apply it to OFL detection in pond water sample.Results:rGO-AuNPs composite nanomaterials was successfully synthesized by using Lilium casa blanca petals biomass as reductants.Gold nanoparticles were observed on reduced graphene oxide sheets and the EF was 3.88×10^(7).Additionally,SERS intensity had a good linear relationship with ofloxacin mass concentration in the range of 1 to 500 ng/mL,and the limit of detection was 0.3 ng/mL.The recovery was 96.28%to 102.84%,and the relative standard deviation was less than 10%.Conclusion:The synthesized rGO-AuNPs had high SERS activity and a positive application prospects in ofloxacin detection.

关 键 词：表面增强拉曼散射 还原氧化石墨烯—金复合纳米材料 核酸适配体 氧氟沙星 

分 类 号：O657.37[理学—分析化学] TS207.3[理学—化学]