检测新生儿溶血标本NSE校正公式的建立  

Establishment of NSE correction formula for detecting hemolytic samples of Neonate

作　　者：常珊碧 张保荣 沈洁 蒋曼丽 王跃帮 CHANG Shanbi;ZHANG Baorong;SHEN Jie;JIANG Manli;WANG Yuebang(Department of Medical Laboratory,Suqian First Hospital,Suqian 223800,Jiangsu,China)

机构地区：[1]宿迁市第一人民医院医学检验科,江苏宿迁223800

出　　处：《西部医学》2023年第9期1396-1399,共4页Medical Journal of West China

基　　金：宿迁市临床医学检验实验室(M201902)。

摘　　要：目的分析溶血对新生儿神经元特异性烯醇化酶(NSE)的影响,建立新生儿溶血NSE校正公式。方法选取50份出生<24 h的新生儿样本,离心分离上层血清,添加反复冻融红细胞悬液,罗氏E602电化学发光检测血清中NSE浓度,应用仪器法和氰化高铁法(HiCN)分别检测血清中血红蛋白(Hb)结果,分别建立NSE校正公式。分析仪器法和HiCN法NSE的浓度变化(ΔNSE)的相关性,评价仪器法NSE校正公式的可行性。结果50份溶血样本ΔNSE为(35.62±2.17)μg/g Hb。以ΔNSE为Y轴、ΔHb_(HiCN)为X轴,血清NSE浓度变化与Hb浓度变化之间的相关方程Y_(ΔNSE)=32.90×Hb全血浓度+1.58(r^(2)=0.991,P<0.001),Y_(ΔNSE)=30.97×HbHICN+1.11(r^(2)=0.981,P<0.001)。仪器法和HiCN法得出NSE结果呈较高相关性(r^(2)=0.933,P<0.01)且两者之间差异无统计学意义(P=0.413)。结论溶血样本严重影响新生儿NSE检测结果。校正后的NSE结果,可以为临床诊断提供参考价值。Objective To analyze the effect of hemolysis on neonatal neuron specific enolase(NSE)and establish the correction formula of NSE.Methods 50 samples of newborn babies less than 24h old were selected.The upper serum was separated by centrifugation,and repeated freeze-thaw red cell suspension was added.The concentration of NSE in serum was detected by Roche E602 electrochemilescence.The serum hemoglobin(Hb)was detected by instrument method and high ferric cyanide method(HiCN).The NSE correction formula was established respectively.The correlation of NSE concentration changes(ΔNSE)between the instrumental method and HiCN method was analyzed,and the feasibility of the NSE correction formula of the instrumental method was evaluated.ResultsΔNSE of 50 hemolytic samples was(35.62±2.17)μg/g Hb.The correlation equation between the change of serum NSE concentration and the change of Hb concentration is Y-axis withΔNSE as the Y-axis andΔHBHICN as the x-axisΔNSE=32.90*Hb whole blood concentration+1.58(r^(2)=0.991,P<0.001),Y_(ΔNSE)=30.97*HbHICN+1.11(r^(2)=0.981,P<0.001).There was a high correlation between NSE results obtained by instrumental method and HiCN method(r^(2)=0.933,P<0.01),and there was no significant difference between them(P=0.413).Conclusion Hemolytic samples seriously affect the detection results of neonatal NSE.The corrected NSE results can provide reference value for clinical diagnosis.

关 键 词：新生儿 溶血样本 校正公式 仪器法 氰化高铁血红蛋白测定法 神经元特异性烯醇化酶 

分 类 号：R772.1[医药卫生—眼科]