Total glucosides of Rhizoma Smilacis Glabrae:a therapeutic approach for psoriasis by regulating Th17/Treg balance  

作　　者：TANG Yingzhan YU Jingyi ZHAO Wen LIU Juyan PENG Hongying ZHANG Haoran JIANG Zhenzhou YU Qinwei ZHANG Luyong 

机构地区：[1]New drug screening center,Jiangsu Center for Pharmacodynamics Research and Evaluation,China Pharmaceutical University,Nanjing 210009,China [2]Guangzhou Baiyunshan Jingxiutang Pharmaceutical Co.,Ltd.,Guangzhou 510130,China [3]Guangzhou Pharmaceutical Holdings Co.,Ltd.,Guangzhou 510103,China [4]Center for Drug Research and Development,Guangdong Pharmaceutical University,Guangzhou 510006,China

出　　处：《Chinese Journal of Natural Medicines》2023年第8期589-598,共10页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.82074114,81973562 and 81773995),the Scholar of the 14th batch of“Six Talents Peak”highlevel talent selection(No.SWYY-094);the“Double First-Class”University project(No.CPU2018GY33).

摘　　要：Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T cells.In this study,we aimed to investigate the potential application of RSG in the treatment of psoriasis and elucidate its mechanism of action and material basis.Our findings revealed significant improvements upon administration of RSG in an imiquimod(IMQ)-induced psoriasis model.These improvements were characterized by a remarkable increase in the number of tail scales in mice and a substantial amelioration of skin erythema,ulceration,and flaking.By transcriptome sequencing and T-cell flow sorting assay,we identified notable effects of RSG on the modulation of various cellular processes.Specifically,RSG prominently down-regulated the Th17/Treg ratio in damaged skin tissues and reduced the proportion of G2 phase cells.Furthermore,RSG exhibited a stimulatory effect on the proliferation and differentiation of epithelial cells.Of particular interest,we discovered thatβ-sitosterol,sitostenone,stigmasterol,smiglanin,and cinchonain Ib displayed potent inhibitory effects on the IL-17-mediated inflammatory response in HaCaT cells.In summary,our study highlights the therapeutic potential of RSG in the treatment of psoriasis,attributed to its ability to regulate the Th17/Treg balance.These findings contribute to the development of new indications for RSG and provide a solid theoretical foundation for further exploration in this field.

关 键 词：PSORIASIS Rhizoma Smilacis Glabrae Th17/Treg balance Inflammation Epithelium development 

分 类 号：R965[医药卫生—药理学]