Expression analyses of stress‑responsive genes in the hermatypic coral Acropora tenuis and its symbiotic dinofagellates after exposure to the herbicide Diuron  

作　　者：Hiroshi Ishibashi Seigo Minamide Ichiro Takeuchi 

机构地区：[1]Graduate School of Agriculture,Ehime University,Matsuyama,Ehime 790-8566,Japan [2]Center of Advanced Technology for the Environment,Graduate School of Agriculture,Ehime University,Matsuyama,Ehime 790-8566,Japan

出　　处：《Marine Life Science & Technology》2023年第3期289-299,共11页海洋生命科学与技术（英文）

基　　金：This work was supported by the Japan Society for the Promotion of Science(JSPS)Grants-in-Aid for Scientifc Research(KAKENHI)(Grant Nos.26281048 and 21H03619).

摘　　要：Diuron is one of the most frequently applied herbicides in sugarcane farming in southern Japan,and Australia.In addition,it is used as a booster substance in copper-based antifouling paints.Due to these various uses,Diuron is released into the marine environment;however,little information is available on gene expression in corals and their symbiotic algae exposed to Diuron.We investigated the efects of Diuron on stress-responsive gene expression in the hermatypic coral Acropora tenuis and its symbiotic dinofagellates.After seven days of exposure to 1µg/L and 10µg/L Diuron,no signifcant changes in the body colour of corals were observed.However,quantitative reverse transcription-polymerase chain reaction analyses revealed that the expression levels of stress-responsive genes,such as heat shock protein 90(HSP90),HSP70,and calreticulin(CALR),were signifcantly downregulated in corals exposed to 10µg/L of Diuron for seven days.Moreover,aquaglyceroporin was signifcantly downregulated in corals exposed to environmentally relevant concentrations of 1µg/L Diuron.In contrast,no such efects were observed on the expression levels of other stress-responsive genes,such as oxidative stress-responsive proteins,methionine adenosyltransferase,and green/red fuorescent proteins.Diuron exposure had no signifcant efect on the expression levels of HSP90,HSP70,or HSP40 in the symbiotic dinofagellates.These results suggest that stress-responsive genes,such as HSPs,respond diferently to Diuron in corals and their symbiotic dinofagellates and that A.tenuis HSPs and CALRs may be useful molecular biomarkers for predicting stress responses induced by the herbicide Diuron.

关 键 词：Acropora tenuis CALRETICULIN Coral bleaching DIURON Heat shock protein 

分 类 号：Q178.53[生物学—水生生物学]