Short‑chain fatty acids(SCFAs)as potential resuscitation factors that promote the isolation and culture of uncultured bacteria in marine sediments  

作　　者：Chun-Shui Sun Liu-Yan Zhou Qi-Yun Liang Xiao-Man Wang Yi-Xuan Lei Zhen-Xing Xu Feng-Qing Wang Guan-Jun Chen Zong-Jun Du Da-Shuai Mu 

机构地区：[1]Marine College,Shandong University,Weihai 264209,China [2]State Key Laboratory of Microbial Technology,Institute of Microbial Technology,Shandong University,Qingdao 266237,China [3]Weihai Research Institute of Industrial Technology of Shandong University,Weihai 264209,China [4]Institute of Microbiology Applications,Xinjiang Academy of Agricultural Sciences,Urumqi 830000,China [5]Tancheng County Inspection and Testing Center,Tancheng 276100,China [6]Department of Applied Biological Chemistry,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Bunkyo-Ku,Tokyo 113-8657,Japan [7]Max Planck Institute for Marine Microbiology,Celsiusstraße 1,28359 Bremen,Germany

出　　处：《Marine Life Science & Technology》2023年第3期400-414,共15页海洋生命科学与技术（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(41876166);Natural Science Foundation of Shandong Province,China(Grant No.ZR2022QD126);Science and Technology Fundamental Resources Investigation Program(Grant No.2022FY101100,2019FY100700).

摘　　要：Many marine bacteria are difcult to culture because they are dormant,rare or found in low-abundances.Enrichment culturing has been widely tested as an important strategy to isolate rare or dormant microbes.However,many more mechanisms remain uncertain.Here,based on 16S rRNA gene high-throughput sequencing and metabolomics technology,it was found that the short-chain fatty acids(SCFAs)in metabolites were signifcantly correlated with uncultured bacterial groups during enrichment cultures.A pure culture analysis showed that the addition of SCFAs to media also resulted in high efciency for the isolation of uncultured strains from marine sediments.As a result,238 strains belonging to 10 phyla,26 families and 82 species were successfully isolated.Some uncultured rare taxa within Chlorobi and Kiritimatiellaeota were successfully cultured.Amongst the newly isolated uncultured microbes,most genomes,e.g.bacteria,possess SCFA oxidative degradation genes,and these features might aid these microbes in better adapting to the culture media.A further resuscitation analysis of a viable but non-culturable(VBNC)Marinilabiliales strain verifed that the addition of SCFAs could break the dormancy of Marinilabiliales in 5 days,and the growth curve test showed that the SCFAs could shorten the lag phase and increase the growth rate.Overall,this study provides new insights into SCFAs,which were frst studied as resuscitation factors in uncultured marine bacteria.Thus,this study can help improve the utilisation and excavation of marine microbial resources,especially for the most-wanted or key players.

关 键 词：Marine bacteria SCFAs VBNC Resuscitation culture 

分 类 号：Q93[生物学—微生物学]