为实现活的非可培养(Viable but nonculturable state, VBNC)态沙门氏菌的定量检测,文章根据沙门氏菌的特异性毒力基因设计引物和探针,优化微滴数字聚合酶链反应(Droplet digital polymerase chain reaction, ddPCR)反应条件及叠氮丙啶(...
supported by the National Natural Science Foundation of China(Nos.41861144023 and U2005206);the Xiamen Municipal Bureau of Science and Technology(No.YDZX20203502000003).
Viable but non-culturable(VBNC)bacteria have been detected in source water and effluent of drinking water treatment processes,leading to significant underestimation of viable cell counts.Limited information exists on ...
This work was supported by the National Natural Science Foundation of China(41876166);Natural Science Foundation of Shandong Province,China(Grant No.ZR2022QD126);Science and Technology Fundamental Resources Investigation Program(Grant No.2022FY101100,2019FY100700).
Many marine bacteria are difcult to culture because they are dormant,rare or found in low-abundances.Enrichment culturing has been widely tested as an important strategy to isolate rare or dormant microbes.However,man...
supported by the National Natural Science Foundation of China(No.42077391);the Guangdong Foundation for Program of Science and Technology Research,China(No.2020B1212060053).
The production and reactivation of viable but nonculturable(VBNC)bacteria by chlorine disinfection leads to an overestimation of the disinfection effect and brings health risks.Therefore,this study is to evaluate the ...