Occurrence of viable but non-culturable(VBNC)pathogenic bacteria in tap water of public places  

作　　者：Lizheng Guo Xinyan Xiao Kassim Chabi Yiting Zhang Jingjing Li Su Yao Xin Yu 

机构地区：[1]China National Research Institute of Food and Fermentation Industries Co.,Ltd.,China Center of Industrial Culture Collection,Beijing 100015,China [2]Key Laboratory of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China [3]College of Environment&Ecology,Xiamen University,Xiamen 361102,China

出　　处：《Frontiers of Environmental Science & Engineering》2024年第3期115-129,共15页环境科学与工程前沿（英文）

基　　金：supported by the National Natural Science Foundation of China(Nos.41861144023 and U2005206);the Xiamen Municipal Bureau of Science and Technology(No.YDZX20203502000003).

摘　　要：Viable but non-culturable(VBNC)bacteria have been detected in source water and effluent of drinking water treatment processes,leading to significant underestimation of viable cell counts.Limited information exists on VBNC bacteria in tap water,particularly in public places.To address this gap,a comprehensive nine-month study was conducted in a major city in south-eastern China,using culture-based and quantitative PCR with propidium monoazide(PMA)dye methods.Forty-five samples were collected from five representative public places(railway station,campus,hospital,shopping mall,and institution).The findings revealed that culturable bacteria represented only 0–17.51%of the viable 16S rRNA genes,suggesting that the majority of viable bacteria existed in an uncultured or VBNC state.Notably,opportunistic pathogens such as Escherichia coli,Enterococcus faecalis,Pseudomonas aeruginosa,Salmonella sp.,and Shigella sp.were primarily detected as VBNC cells,with concentrations ranging from 1.03×10^(0)to 3.01×10^(3),1.20×10^(0)to 1.42×10^(2),1.32×10^(0)to 8.82×10^(0),1.00×10^(0)to 6.71×10^(1),and 2.07×10^(0)to 1.93×10^(2)cell equivalent/100 mL,respectively.Culturable P.aeruginosa was observed in tap water after prolonged stagnation,indicating potential risks associated with bacterial regrowth.Spatial and temporal factors accounted for 17.1%and 26.0%,respectively,of the variation in tap water community structure during the sampling period,as revealed by 16S rRNA amplicon sequencing.This study provides quantitative insights into the occurrence of VBNC bacteria in tap water and highlights the need for more sensitive monitoring methods and microbial control techniques to enhance tap water safety in public locations.

关 键 词：VBNC Pathogenic bacteria PMA treatment Public tap water Community structure 

分 类 号：Q93[生物学—微生物学]