HPLC法同时测定不同品种、产地、部位松树中莽草酸的含量  

Simultaneous Determination of the Content of Shikimic Acid in Pine Trees from Different Varieties,Origins,and Parts by HPLC

作　　者：刘东彦[1] 石晓峰[1] 王信 王新娣[1] 马趣环[1] 张家旭 LIU Dong-yan;SHI Xiao-feng;WANG Xin;WANG Xin-di;MA Qu-huan;ZHANG Jia-xu(Gansu Academy of Medical Science,Lanzhou 730050,Gansu,P.R.China;Gansu University of Chinese Medicine,Lanzhou 730000,Gansu,P.R.China)

机构地区：[1]甘肃省医学科学研究院,甘肃兰州730050 [2]甘肃中医药大学,甘肃兰州730000

出　　处：《林产工业》2023年第9期39-43,共5页China Forest Products Industry

基　　金：甘肃省科技支撑计划项目(1204FKCA152);甘肃省中医药重点科研项目(GZKZ-2020-6);2019年甘肃省省级引导科技创新发展专项资金(17)。

摘　　要：研究采用HPLC法同时测定不同品种、产地、部位松树中莽草酸的含量,以期为开发莽草酸资源提供参考。色谱柱为Agilent Eclipse Plus C_(18)(250 mm×4.6 mm,5 m),流动相为甲醇/0.1%磷酸水溶液(体积比为10∶90),检测波长为217 nm,柱温为30℃。采用直观分析结合聚类分析对不同品种、产地、部位松树中莽草酸含量的差异性进行统计分析。结果表明:莽草酸的线性关系良好(r=0.9995),平均加样回收率为100.4%(n=6),RSD为1.71%。6种松树中的莽草酸含量由高到低依次为雪松、华山松、白皮松、油松、樟子松、落叶松。其中,天水雪松松针中的莽草酸含量最高,达31.47 mg/g。通过聚类分析发现,6种松树中的莽草酸可划分为3类。In this study,HPLC was used to determine the content of shikimic acid in pine trees from different varieties,origins,and parts,in order to provide reference for its further development and application.HPLC test was performed on a 30℃Agilent Eclipse Plus C18 column(250 mm×4.6 mm,5μm),with the mobile phase comprising of methanol/0.1%phosphoric acid solution(10∶90,V/V),and the detection wavelength was set at 217 nm.The differences in the content of shikimic acid were analyzed by visual analysis and cluster analysis methods.The results showed that shikimic acid was linearly good(r=0.9995),with the average recovery rate of 100.4%(n=6)and the RSD of 1.71%.The content of shikimic acid was in six pine trees from high to low was Cedrus deodara(Roxb)G.Don,Pinus armandi Franch.,Pinus bungeana Zucc.ex Endl.,Pinus tabulaeformis Carr.,Larix gmelinii(Rupr.)Kuzen.,and P.sylvestris var.mongolica Litv.,in which C.deodara pine needles from Tianshui had the highest content at 31.47 mg/g.The shikimic acid in six pine trees can be divided into three categories by cluster analysis.

关 键 词：松针 松枝 莽草酸 含量测定 聚类分析 

分 类 号：TS6[轻工技术与工程] TS396