早期胃癌及癌前病变miRNA-mRNA网络相关性及调控机制  

Network correlation of miRNA-mRNA and the regulatory mechanism in early gastirc cancer and gastric precancerous lesions

作　　者：程捷瑶 陆星华[2] 吴晰[2] 冯林[3] 崔建芳[2] 于航[2] 杨爱明[2] CHENG Jieyao;LU Xinghua;WU Xi;FENG Lin;CUI Jianfang;YU Hang;YANG Aiming(Department of Gastroenterology,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038;Department of Gastroenterology,Peking Union Medical College Hospital;State Key Laboratory of Molecular Oncology,Department of Etiology and Carcinogenesis,National Cancer Center/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,China)

机构地区：[1]首都医科大学附属北京世纪坛医院消化内科,北京100038 [2]北京协和医院消化内科 [3]中国医学科学院肿瘤医院分子肿瘤学国家重点实验室

出　　处：《胃肠病学和肝病学杂志》2023年第9期967-971,共5页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的分析早期胃癌、高级别上皮内瘤变和慢性非萎缩性胃炎组织的mRNA和miRNA表达谱特征,为了解胃癌的发生机制提供线索。方法运用mRNA和miRNA表达谱芯片技术对19例早期胃癌、18例高级别上皮内瘤变和11例慢性非萎缩性胃炎组织进行分析,寻找差异基因,并通过GO富集分析了解其生物学功能。通过整合分析miRNA及mRNA表达谱芯片数据,构建与胃癌发生相关的miRNA-mRNA表达调控网络。结果高级别上皮内瘤变与早期胃癌组织mRNA表达谱特征较相似,均与慢性非萎缩性胃炎组存在显著差异。在高级别上皮内瘤变和早期胃癌患者组织中表达上调的基因主要参与炎症、免疫反应。早期胃癌组与慢性非萎缩性胃炎组miRNA表达谱差异显著,与慢性非萎缩性胃炎相比,有24个miRNA在早期胃癌中低表达,其调控的668个mRNA在早期胃癌中高表达,这些mRNA主要生物学功能集中在与炎症、免疫反应及与癌症相关的信号通路上。结论胃癌变早期已出现基因表达谱改变,免疫、炎症反应是胃癌发生的早期事件,在这一过程中miRNA的表达调控可能起了重要作用。Objective To analyze the mRNA and miRNA expression profile of patients with early gastric cancer(EGC),high-grade intraepithelial neoplasia(HGIN)and chronic non-atrophic gastritis(CNAG),and to find the molecular and functional changes in early carcinogenesis.Methods The mRNA and miRNA expression profiling was explored on 19 EGC,18 HGIN and 11 CNAG samples with Agilent microarrays.We integrated the information from the molecular levels of miRNA and mRNA,and built the miRNA-mRNA correlation molecular network.To find the related important genes in gastric early carcinogenesis,GO enrichment analysis was performed to explore the molecular functional of the important genes.Results The gene expression patterns of HGIN and EGC tissues were similar,but were significant different with CNAG tissues.The related biological function of the over-expressed genes in HGIN and EGC tissues were mainly focused on the inflammation response and immune response.By integrating the information from the molecular levels of miRNA and mRNA,we built the miRNA-mRNA correlation molecular network and found that the gene expression patterns of EGC was significant different with CNAG tissues.The biological function of the up-regulated genes in EGC were in the category of immune response,inflammation response,and cancer.Conclusion Immune response and inflammation response contribute to the malignant progression of gastric neoplasia.

关 键 词：基因表达谱芯片 整合分析 分子网络 早期胃癌 癌前病变 

分 类 号：R735.2[医药卫生—肿瘤]