基于转录组分析金属离子对绿绒蒿属3种植物花瓣呈色的影响  

Analysis on effects of metal ions on the petal color of three species in Meconopsis based on transcriptome

作　　者：罗军 陈丽琦 李拓键 区智[1] 屈燕[1] LUO Jun;CHEN Liqi;LI Tuojian;OU Zhi;QU Yan(Southwest Research Center for Engineering Technology of Landscape Architecture(State Forestry and Grassland Administration),Yunnan Engineering Research Center for Functional Flower Resources and Industrialization,College of Landscape Architecture and Horticulture Sciences,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学园林园艺学院国家林业和草原局西南风景园林工程技术研究中心云南省功能性花卉资源及产业化技术工程研究中心,云南昆明650224

出　　处：《植物资源与环境学报》2023年第5期16-27,共12页Journal of Plant Resources and Environment

基　　金：国家自然科学基金项目(32160404,31460218);云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-211)。

摘　　要：为探究金属离子对绿绒蒿属(Meconopsis Vig.)3种植物花瓣呈色的影响,测定了盛开期全缘叶绿绒蒿(M.integrifolia(Maxim.)Franch.)、红花绿绒蒿(M.punicea Maxim.)和川滇绿绒蒿(M.wilsonii Grey-Wilson)花瓣中7种金属离子(包括Fe^(3+)、Mg^(2+)、Ca^(2+)、K^(+)、Mn^(2+)、Cu^(2+)和Zn^(2+))含量,对3个花期花瓣进行转录组分析。结果表明:盛开期,全缘叶绿绒蒿花瓣中7种金属离子含量显著(P<0.05)高于红花绿绒蒿和川滇绿绒蒿;红花绿绒蒿花瓣中Fe^(3+)、Mg^(2+)、K^(+)、Mn^(2+)和Zn^(2+)含量显著低于川滇绿绒蒿,Cu^(2+)和Ca^(2+)含量显著高于川滇绿绒蒿。全缘叶绿绒蒿花瓣中,Fe^(3+)、Fe^(2+)和Cu^(2+)相关GO富集条目以及镁螯合酶活性、钾离子稳态和对锌离子的反应条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中总体最多,锌离子结合和锰离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最少,螯合钙离子释放到细胞溶质条目中仅全缘叶绿绒蒿花瓣的差异unigene明显表达。红花绿绒蒿花瓣中,亚铁结合、镁离子结合、钙离子结合、钾离子结合、钾离子转运条目和锌离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最多,仅三价铁结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最少。川滇绿绒蒿花瓣中,细胞钙离子稳态和锰离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中最多,Mg^(2+)、K^(+)和Cu^(2+)相关GO富集条目以及亚铁结合、铁离子的细胞内螯合、对锌离子的反应、钙离子结合条目存在明显表达的差异unigene数量在供试绿绒蒿属3种植物中总体最少。综上所述,推测Cu^(2+)和Ca^(2+)影响全缘叶绿绒蒿花瓣呈色,Fe^(2+)、Mg^(2+)、Mn^(2+)、K^(+)和Cu^(2+)影响红花绿绒蒿花瓣呈色,Fe^(3+)、Mg^(2+)、Mn^(2+)和Cu^(2+)影响川滇绿绒蒿花瓣呈色�In order to explore the effects of metal ions on the petal color of three species in Meconopsis Vig.,contents of seven metal ions(including Fe^(3+),Mg^(2+),Ca^(2+),K^(+),Mn^(2+),Cu^(2+),and Zn^(2+))in petals of M.integrifolia(Maxim.)Franch.,M.punicea Maxim.,and M.wilsonii Grey-Wilson at the blooming stage were measured,and transcriptome analyses were conducted for petals at three flowering stages.The results show that at the blooming stage,contents of seven metal ions are significantly(P<0.05)higher in petals of M.integrifolia than in petals of M.punicea and M.wilsonii;contents of Fe^(3+),Mg^(2+),K^(+),Mn^(2+),and Zn^(2+)in petals of M.punicea are significantly lower than those of M.wilsonii,and contents of Cu^(2+)and Ca^(2+)are significantly higher than those of M.wilsonii.In petals of M.integrifolia,evidently expressed differential unigenes in Fe^(3+)-,Fe^(2+)-,and Cu^(2+)-related GO enrichment items,and magnesium chelatase activity,potassium homeostasis and response to zinc ion items are the most among three test species in Meconopsis in general,while those in zinc ion binding and manganese ion binding items are the least,and differential unigenes in the release of sequestered calcium ion into cytosol item only evidently express in petals of M.integrifolia.In petals of M.punicea,evidently expressed differential unigenes in ferrous iron binding,magnesium ion binding,calcium ion binding,potassium ion binding,potassium ion transport,and zinc ion binding items are the most among three test species in Meconopsis,and only those in ferric iron binding item are the least among three test species in Meconopsis.In petals of M.wilsonii,evidently expressed differential unigenes in cellular calcium ion homeostasis and manganese ion binding items are the most among three test species in Meconopsis,and those in Mg^(2+)-,K^(+)-and Cu^(2+)-related GO enrichment items,and ferrous iron binding,intracellular sequestering of iron ion,response to zinc ion and calcium ion binding items are the least among three test species in Mecon

关 键 词：绿绒蒿属 花色 金属离子 差异unigene 基因表达 

分 类 号：Q786[生物学—分子生物学] S68[农业科学—观赏园艺]