黄芩苷调节Notch/JAG1信号通路对舌癌细胞恶性生物学行为的影响  

Impact of Baicalin on Malignant Biological Behavior of Tongue Cancer Cells by Regulating Notch/JAG1 Signaling Pathway

作　　者：王欢 夏力[1] 李玮[1] 谢春凤 WANG Huan;XIA Li;LI Wei(Mianyang Central Hospital,Sichuan Mianyang 621000,China)

机构地区：[1]四川省绵阳市中心医院耳鼻咽喉颌面外科,四川绵阳621000

出　　处：《河北医学》2023年第9期1438-1443,共6页Hebei Medicine

基　　金：2019年四川省卫生健康委员会科研课题(重点研究项目),(编号19ZD016)。

摘　　要：目的:探讨黄芩苷(Bai)调节Notch/JAG1信号通路对舌癌细胞恶性生物学行为的影响。方法:用0、10、20、30、40、50和100μM Bai处理人口腔上皮细胞HOEC。将人舌磷癌细胞CAL27分为CAL27组(未处理的CAL27细胞)、L-Bai组(10μM Bai处理CAL27细胞)、M-Bai组(20μM Bai处理CAL27细胞)、H-Bai组(40μM Bai处理CAL27细胞)、VPA组(8mM Notch/JAG1通路激活剂VPA处理CAL27细胞)、H-Bai+VPA组(40μM Bai和8mM VPA共同处理CAL27细胞);MTT法检测Bai对人口腔上皮细胞HOEC细胞毒性和CAL27细胞活力的影响;流式细胞术检测CAL27细胞凋亡;Transwell检测CAL27细胞侵袭、迁移能力;Western blot检测上皮-间充质转化(EMT)蛋白以及Notch/JAG1信号通路蛋白表达。结果:0~100μM Bai对HOEC细胞无明显毒性影响。与CAL27组相比,L-Bai组、M-Bai组、H-Bai组OD值、细胞迁移、侵袭数量、vimentin、Snail蛋白水平、Notch1、JAG1蛋白水平依次显著下降(P<0.05),凋亡率、E-cadherin蛋白水平依次显著升高(P<0.05),而VPA组以上指标趋势相反;与H-Bai组相比,H-Bai+VPA组OD值、细胞迁移、侵袭数量、vimentin、Snail蛋白水平、Notch1、JAG1蛋白水平显著增加(P<0.05),凋亡率、E-cadherin蛋白水平显著减少(P<0.05)。结论:Bai可能通过抑制Notch/JAG1信号通路对舌癌细胞增殖、凋亡和EMT产生影响。Objective:To investigate the impact of baicalin(Bai)on the malignant biological behavior of tongue cancer cells by regulating Notch/JAG1 signal pathway.Methods:Human oral epithelial cells HOEC was treated with 0,10,20,30,40,50 and 100μM Bai.Human tongue phosphorous carcinoma cell line CAL27 was grouped into CAL27 group(untreated CAL27 cells),L-Bai group(10μM Bai treated CAL27 cells),M-Bai group(20μM Bai treated CAL27 cells),H-Bai group(40μM Bai treated CAL27 cells),VPA group(8 mM Notch/JAG1 pathway activator VPA treated CAL27 cells),and H-Bai+VPA group(40μM Bai and 8 mM VPA co-treated CAL27 cells);MTT assay was applied to detect the effects of Bai on HOEC cytotoxicity and CAL27 cell viability;the apoptosis of CAL27 cells was detected by flow cytometry;Transwell was applied to detect the invasion and migration abilities of CAL27 cells;Western blot was applied to detect the expression of epithelial-mesenchymal transformation(EMT)protein and Notch/JAG1 signaling pathway protein.Results:0~100μM Bai had no obvious toxic effect on HOEC cells.Compared with CAL27 group,the OD value,cell migration number,invasion number,the protein levels of vimentin,Snail,the protein levels of Notch1 and JAG1 in L-Bai group,M-Bai group and H-Bai group decreased obviously in turn(P<0.05),the apoptosis rate and the level of E-cadherin protein increased obviously in turn(P<0.05),in the VPA group,the above indicators had the opposite trends;compared with H-Bai group,the OD value,cell migration number,invasion number,the protein levels of vimentin,Snail,the protein levels of Notch1 and JAG1 in H-Bai+VPA group increased obviously(P<0.05),the apoptosis rate and the level of E-cadherin protein decreased obviously(P<0.05).Conclusion:Bai may affect the proliferation,apoptosis and EMT of tongue cancer cells by inhibiting Notch/JAG1 signaling pathway.

关 键 词：黄芩苷 Notch/JAG1信号通路 舌癌 增殖 凋亡:迁移 

分 类 号：R739.8[医药卫生—肿瘤]