基质金属蛋白酶7(MMP7)和硫酸乙酰肝素糖蛋白2(HSPG2)在胰腺癌中的表达及临床意义  

Expression and clinical significance of matrix metalloproteinase 7 and heparan sulfate proteoglycan 2 in pancreatic cancer

作　　者：陈金凤 陆小柳 张晓韵 唐曦平 CHEN Jinfeng;LU Xiaoliu;ZHANG Xiaoyun;TANG Xiping(School of Medical Oncology,Guangxi Medical University,Nanning 530021,China;Endoscopy Center,Guangxi Medical University Cancer Hospital,Nanning 530021,China)

机构地区：[1]广西医科大学肿瘤医学院,南宁530021 [2]广西医科大学附属肿瘤医院内镜中心,南宁530021

出　　处：《临床肝胆病杂志》2023年第9期2169-2174,共6页Journal of Clinical Hepatology

基　　金：广西自然科学基金(2020GXNSFAA297062);广西医疗卫生适宜技术开发与推广应用项目(S2020103);广西壮族自治区中医药管理局科研项目(GZZC2020235)。

摘　　要：目的探讨基质金属蛋白酶7(MMP7)和硫酸乙酰肝素糖蛋白2(HSPG2)在胰腺癌患者中的表达及临床意义。方法选取2018年1月—2020年12月广西医科大学附属肿瘤医院收治的胰腺癌患者30例,收集胰腺癌组织标本及其血清样本,同时收集癌旁正常组织以及30例健康志愿者血清样本作为对照。采用免疫组化方法检测胰腺癌组织及癌旁正常组织中MMP7和HSPG2蛋白表达;采用酶联免疫吸附法检测胰腺癌患者和健康对照者血清样本中MMP7和HSPG2的表达水平;收集患者临床资料并分析MMP7和HSPG2表达与临床病理特征、生存预后的关系。计量资料两组间比较采用成组t检验。计数资料组间比较采用χ^(2)检验或Fisher确切概率法。MMP7与HSPG2表达的关系采用Spearman相关分析。采用Kaplan-Meier法绘制生存曲线,生存情况比较采用Log-rank检验。结果免疫组化结果显示,胰腺癌组织中MMP7和HSPG2蛋白阳性表达均高于癌旁组织,差异均有统计学意义(χ^(2)值分别为31.093、35.623,P值<0.05)。胰腺癌患者血清中MMP7、HSPG2水平均显著高于健康对照组(t值分别为20.174、32.600,P值<0.05)。MMP7和HSPG2表达均与肿瘤直径、TNM分期有关(P值<0.05)。Spearman相关性分析显示,HSPG2与MMP7之间的表达呈正相关(r=0.539,P=0.002)。MMP7和HSPG2蛋白阳性表达患者总生存率明显低于阴性对照患者(χ^(2)值分别为4.084、12.554,P值均<0.05)。结论MMP7和HSPG2在胰腺癌组织和血清中呈阳性表达,且与肿瘤的直径、TNM分期有关,两者可能在胰腺癌的发生发展中存在某种关联,有望成为胰腺癌诊断和判断预后的潜在标志物。Objective To investigate the expression and clinical significance of matrix metalloproteinase 7(MMP7)and heparan sulfate proteoglycan 2(HSPG2)in patients with pancreatic cancer.Methods Pancreatic cancer tissue samples,normal adjacent tissue samples,and serum samples were collected from 30 patients who were admitted to Guangxi Medical University Cancer Hospital from January 2018 to December 2020,and serum samples were collected from 30 healthy volunteers as controls.Immunohistochemistry was used to measure the protein expression levels of MMP7 and HSPG2 in pancreatic cancer tissue samples and normal adjacent tissue samples;ELISA was used to measure the expression levels of MMP7 and HSPG2 in the serum samples of the pancreatic cancer patients and the healthy controls;related clinical data were collected to analyze the association of the expression of MMP7 and HSPG2 with clinicopathological features,survival,and prognosis.The independent-samples t test was used for comparison of continuous data between two groups,and the chi-square test or the Fisher’s exact test was used for comparison of categorical data between two groups;the Spearman correlation analysis was used to investigate the correlation between MMP7 and HSPG2;the Kaplan-Meier method was used to plot survival curves,and the Log-rank test was used for survival analysis.Results Immunohistochemistry showed that the protein expression levels of MMP7 and HSPG2 in pancreatic cancer tissue were significantly higher than those in adjacent tissue(χ^(2)=31.093 and 35.623,both P<0.05).The patients with pancreatic cancer had significantly higher serum levels of MMP7 and HSPG2 than the healthy controls(t=20.174 and 32.600,both P<0.05).The expression of MMP7 and HSPG2 was associated with tumor diameter and TNM stage(both P<0.05).The Spearman correlation analysis showed a positive correlation between HSPG2 and MMP7(r=0.539,P=0.002).The patients with positive protein expression of MMP7 and HSPG2 had a significantly lower overall survival rate than those with negative e

关 键 词：胰腺肿瘤 基质金属蛋白酶7 类肝素硫酸蛋白聚糖类 

分 类 号：R735.9[医药卫生—肿瘤]