苦荞种质资源芦丁含量与SSR标记关联分析  

Association Analysis of Rutin Content with SSRMarkers in Tartary Buckwheat Germplasm Resources

作　　者：吴怡超 李晓瑜 何星星 岳林清 崔静斌 丁梦琦 张建[1] 易泽林[1] 方小梅 WU Yichao;LI Xiaoyu;HE Xingxing;YUE Linqing;CUI Jingbin;DING Mengqi;ZHANG Jian;YI Zelin;FANG Xiaomei(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2023年第9期2-11,共10页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市自然科学基金项目(CSTB2022NCQ-msx1034);重庆市农委荞麦技术产业体系项目(202201).

摘　　要：芦丁是一种黄酮类化合物,具有强化血管、抗氧化等生物活性.苦荞籽粒富含芦丁,是重要的药食兼用作物.了解和研究苦荞芦丁含量的遗传规律,开发与苦荞籽粒芦丁含量相关的分子标记对选育高芦丁苦荞种质具有重要意义.本研究以263个苦荞种质资源为材料,检测并统计分析芦丁含量,263个种质籽粒芦丁含量的变幅为0.46%~1.43%,变异系数为21.64%,利用实验室已有的77对SSR标记检测结果,聚类分析将263份苦荞资源分为3个类群,群体结构分析将263份种质划分为3个亚群.亲缘关系分析结果说明材料间亲缘关系较远,对关联分析影响较小.GLM和MLM模型的p值QQ plot图显示两个模型都适用于263份苦荞资源的芦丁关联分析,结果表明:TatG0085,TatG0131,TatG0155,TatG0156,TatG0164,TatG0187,TatG0188,S6763,SWU_Ft029,SWU_Ft177,SWU_Ft394,SWU_Ft420这12对SSR标记与苦荞芦丁含量显著关联,其中TatG0164和SWU_Ft394在两个模型中均与芦丁含量呈显著性关联,与前人研究相比,8个标记可能存在一因多效的功能.该研究结果可以为分子标记辅助选择高芦丁苦荞种质资源及苦荞芦丁含量重要功能基因挖掘提供重要科学依据.Rutin a flavonoid compound known for its ability to strengthen blood vessels,antioxidant and other biological activities.Tartary buckwheat is an important medicinal and food crop,which is rich in rutin in its grains.Therefore,it is of great practical significance to understand and study the genetic mechanism of rutin content in Tartary buckwheat and develop markers related to rutin content in Tartary buckwheat grains for breeding high-rutin Tartary buckwheat germplasm.In this study,263 Tartary buckwheat germplasm resources were used as materials to determine and analyzerutin content.The variation range of rutin content was 0.46%to 1.43%and variation coefficient was 21.64%.Based on the genotypes of 263 germplasm resources detected by 77 SSR markers in our previous study,263 Tartary buckwheat germplasm resources were divided into 3 groups by cluster analysis.Similarly,263 germplasm resources were divided into three subgroups by population structure analysis.The relationship analysis showed that the genetic relationship among the 263 germplasm resources was very distant,which had little influence on the association analysis.The QQ plots of GLM and MLM showed that the two models were suitable for the association analysis of rutin of 263 Tartary buckwheat resources.The results show that twelve pairs of SSR markers,TatG0085,TatG0131,TatG0155,TatG0156,TatG0164,TatG0187,TatG0188,S6763,SWU_Ft029,SWU_Ft177,SWU_Ft394,SWU_Ft420,had significant correlations with rutin content in Tartary buckwheat.Among them,TatG0164 and SWU_Ft394 showed significant correlation with rutin content in both models.Compared with previous studies,8 markers may have pleiotropic function.This study provides important scientific basis for molecular marker-assisted selection of high-rutin Tartary buckwheat germplasm resources and mining important functional genes controllingrutin content in Tartary buckwheat.

关 键 词：苦荞 关联分析 芦丁含量 SSR标记 

分 类 号：S517[农业科学—作物学]