利用体外三步法研究驴粗饲料原料的体外营养物质降解率  

Evaluation on nutrient degradation rate of donkey by in vitro three-step method

作　　者：郭蒙霞 刘畅 史彬林[1] 郭晓宇[1] 赵艳丽[1] 郭咏梅[1] 郑亚光[1] 闫素梅[1] GUO Meng-xia;LIU Chang;SHI Bin-lin;GUO Xiao-yu;ZHAO Yan-li;GUO Yong-mei;ZHENG Ya-guang;YAN Su-mei

机构地区：[1]内蒙古农业大学动物科学学院、内蒙古自治区高校动物营养与饲料科学重点实验室,内蒙古呼和浩特010018

出　　处：《饲料研究》2023年第17期99-103,共5页Feed Research

基　　金：内蒙古自治区科技重大专项资助(项目编号:zdzx2018001)。

摘　　要：试验采用体外三步法(胃蛋白酶-胰酶-盲肠液法)研究了6种常用驴粗饲料原料体外营养物质降解率。采用单因素完全随机试验设计,比较研究全株玉米青贮、玉米秸秆、谷草、苜蓿、燕麦草、羊草6种粗饲料的体外营养物质降解率,每种粗饲料作为一个处理,每个处理6个重复。饲料原料在模拟胃内环境消化1 h,模拟小肠内环境消化24 h,在体外盲肠环境连续培养48 h。结果显示,全株玉米青贮和苜蓿在驴胃-小肠体外干物质(DM)、有机质(OM)、粗蛋白(CP)营养物质降解率显著高于玉米秸秆、燕麦草、谷草和羊草(P<0.05)。全株玉米青贮和苜蓿在驴胃-小肠-盲肠体外DM、OM、中性洗涤纤维(NDF)、和半纤维素(HCL)降解率与消化能(DE)均显著高于其他4种粗饲料(P<0.05),苜蓿在驴胃-小肠-盲肠体外CP降解率最高,显著高于其他粗饲料(P<0.05),全株玉米青贮与燕麦草次之。研究表明,6种粗饲料中苜蓿和全株玉米青贮体外营养物质降解率较高,表明其营养价值高于谷草、玉米秸秆、燕麦草和羊草。The study was to study the effect of six common roughage raw materials for donkeys on nutrient degradation rate in vitro by a three-step in vitro method(pepsin-trypsin-cecal fluid method).The single factor randomized design was used to compare the nutrient degradation rates of six kinds of roughages(whole plant corn silage,corn straw,cereal grass,alfalfa,oat grass and Leymus chinensis)in vitro.Each roughage was treated as a treatment,with six replicates in each group.The roughages were digested in simulated stomach environment for 1 h and small intestine environment for 24 h,and then continuously cultured in external cecum environment for 48 h.The results showed that the degradation rates of DM,OM and CP in the stomach and small intestine of whole plant corn silage and alfalfa by donkey were significantly higher than those of corn straw,oat grass,cereal grass and Leymus chinensis(P<0.05).The degradation rates of DM,OM,NDF and HCL and DE in vitro of whole plant corn silage and alfalfa were significantly higher than those of the other four roughages(P<0.05).The degradation rate of CP of alfalfa was the highest,which was significantly higher than that of other roughages(P<0.05),followed by whole plant corn silage and oat grass.The experiment indicates that the whole plant corn silage and alfalfa in fodder have better cecal fermentation effect in vitro,suggesting that their nutritional value is higher than that of cereal grass,corn straw,oat grass and Leymus chinensis.

关 键 词：驴 粗饲料 营养价值评价 胃蛋白酶-胰酶-盲肠液法 

分 类 号：S822[农业科学—畜牧学]