有氧运动训练影响阿尔茨海默症小鼠海马Notch1、Caspase-3的表达  

Effects of aerobic exercise on the expression of Notch1 and Caspase-3 in the hippocampus of Alzheimer’s disease mice

作　　者：杨力源[1] 张业廷 李垂坤[1] 魏翠兰[3] Yang Liyuan;Zhang Yeting;Li Chuikun;Wei Cuilan(College of Physical Education,Chengdu University,Chengdu 610106,Sichuan Province,China;Civil Aviation Flight University of China,Guanghan 618307,Sichuan Province,China;Sports Institute of Chengdu University of Technology,Chengdu 610059,Sichuan Province,China)

机构地区：[1]成都大学体育学院,四川省成都市610106 [2]中国民用航空飞行学院,四川省广汉市618307 [3]成都理工大学体育学院,四川省成都市610059

出　　处：《中国组织工程研究》2024年第26期4113-4120,共8页Chinese Journal of Tissue Engineering Research

基　　金：四川省科技计划项目(2020YFH0184),项目负责人:张业廷;中央高校基本科研业务费专项资金资助(PHD2023-003),项目负责人:张业廷。

摘　　要：背景:β-淀粉样蛋白和Tau蛋白会对阿尔茨海默症患者的认知功能产生不良影响,研究发现Notch1及Caspase-3能够调控β-淀粉样蛋白和Tau蛋白的表达。Notch1及Caspase-3是否介导了有氧运动改善阿尔茨海默症患者认知能力的过程还不清楚,目前缺乏长期有氧运动影响阿尔茨海默症小鼠海马中Notch1及Caspase-3表达的研究。目的:观察长期有氧运动干预阿尔茨海默症小鼠的空间学习记忆情况及其海马中Notch1及Caspase-3的表达,探讨Notch1及Caspase-3对阿尔茨海默症小鼠的影响。方法:将3月龄野生型及APP/PS1双转基因阿尔茨海默症小鼠随机分为4组:野生对照组、野生运动组、阿尔茨海默症对照组、阿尔茨海默症运动组,每组20只。对照组小鼠不进行运动,运动组小鼠进行5个月的有氧运动干预。运动干预结束后,采用Morris水迷宫检测小鼠空间学习记忆能力;采用Real-timePCR、免疫荧光及Westernblot检测各组小鼠海马组织Aβ_(1-42)、Tau、Notch1及Caspase-3蛋白的表达。结果与结论:①阿尔茨海默症小鼠空间学习记忆能力显著差于野生组(P<0.05);运动组小鼠空间学习记忆能力显著优于对照组(P<0.05);②阿尔茨海默症对照组小鼠海马Aβ_(1-42)、Tau、Notch1及Caspase-3表达均显著高于野生对照组(P<0.05);阿尔茨海默症运动组小鼠海马Aβ_(1-42)、Tau、Notch1及Caspase-3表达显著低于阿尔茨海默症对照组(P<0.05);③提示:长期有氧运动干预能够改善阿尔茨海默症小鼠的空间学习记忆能力,而这可能与有氧运动降低阿尔茨海默症小鼠海马Notch1、Caspase-3、Aβ_(1-42)及Tau蛋白表达有关。BACKGROUND:β-amyloid protein and Tau protein have adverse effects on the cognitive function of Alzheimer’s disease patients,and Notch1 and Caspase-3 can regulate the expression of β-amyloid protein and Tau protein.It is not clear whether Notch1 and Caspase-3 mediate the process of aerobic exercise to improve the cognitive ability of Alzheimer’s disease patients.At present,there is a lack of studies on the effect of long-term aerobic exercise on the expression of Notch1 and Caspase-3 in the hippocampus of Alzheimer’s disease mice.OBJECTIVE:To observe the expression of Notch1 and Caspase-3 in the hippocampus of Alzheimer’s disease mice undergoing long-term aerobic exercise and to investigate the effects of Notch1 and Caspase-3 in Alzheimer’s disease mice.METHODS:Wild type and APP/PS1 double-transgenic Alzheimer’s disease mice aged 3 months were randomly divided into four groups:wild control group,wild exercise group,Alzheimer’s disease control group and Alzheimer’s disease exercise group,with 20 mice in each group.Mice in the control groups were not subjected to exercise,while those in the exercise groups received aerobic exercise intervention for 5 months.After the exercise intervention,Morris water maze was used to detect the spatial learning and memory ability of mice.Real-time PCR,immunofluorescence and western blot were used to detect the expressions of Aβ_(1-42),Tau,Notch1 and Caspase-3 in the hippocampal tissues of mice in each group.RESULTS AND CONCLUSION:The spatial learning and memory ability of Alzheimer’s mice was significantly worse than that of wild-type mice(P<0.05).The spatial learning and memory ability of mice in the exercise groups were significantly better than that in the corresponding control groups(P<0.05).The expressions of Aβ_(1-42),Tau,Notch1 and Caspase-3 in the hippocampus were significantly higher in the Alzheimer’s disease control group than the wild control group(P<0.05)and were significantly lower in the Alzheimer’s disease exercise group than the Alzheimer�

关 键 词：阿尔茨海默症 有氧运动 学习记忆能力 NOTCH1 CASPASE-3 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] R741.05