改良Feeney自由落体法建立大鼠创伤性脑损伤模型  

Establishment of a rat model of traumatic brain injury using the modified Feeney’s free-fall method

作　　者：高思淼 吴晓光[1] 韩雪 许士奇[2,4] 李葵花[1,2,4] 彭勇 Gao Simiao;Wu Xiaoguang;Han Xue;Xu Shiqi;Li Kuihua;Peng Yong(Key Laboratory of Nerve Injury and Repair of Hebei Province,Chengde Medical University,Chengde 067000,Hebei Province,China;Department of Biomedical Engineering,Chengde Medical University,Chengde 067000,Hebei Province,China;International Research Center for Medical-Industrial Integration of Hebei Province,Chengde Medical University,Chengde 067000,Hebei Province,China;Department of Neurology,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China;School of Electrical Engineering,Yanshan University,Qinhuangdao 066004,Hebei Province,China;Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province,Yanshan University,Qinhuangdao 066004,Hebei Province,China)

机构地区：[1]承德医学院河北省神经损伤与修复重点实验室,河北省承德市067000 [2]承德医学院生物医学工程系,河北省承德市067000 [3]承德医学院河北省医工结合国际研究中心,河北省承德市067000 [4]承德医学院附属医院神经内科,河北省承德市067000 [5]燕山大学电气工程学院,河北省秦皇岛市066004 [6]燕山大学河北省智能康复及神经调控重点实验室,河北省秦皇岛市066004

出　　处：《中国组织工程研究》2024年第26期4164-4169,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省神经损伤与修复重点实验室开放课题(NJKF202305),项目负责人:李葵花;河北省卫健委医学科学研究课题(20231558),项目负责人:吴晓光;国家自然科学基金(81871029)。

摘　　要：背景:目前关于Feeney自由落体创伤性脑损伤模型,探讨减轻开颅过程中持续磨骨损伤因素的文献较少。目的:通过改变开颅骨窗方式改良创伤性脑损伤大鼠模型。方法:将36只SD大鼠随机平均分成假手术组、模型组和改良模型组。改良模型组造模过程中改良了开骨窗的方式,用小钻头磨骨钻低速2 m/s,在打击的损伤区域边缘打6-8个小孔,每圆孔直径为0.3-0.5 mm,感到空腔而不触及脑实质后立即抽出。改良模型组和假手术组采用改良后的方法开骨窗,模型组按常规开骨窗方法,实施的打击参数相同改良模型组;假手术组不实施打击。造模后第1天对大鼠进行mNSS评分;造模后第1,7天扫描T2WI磁共振成像,测量T2值;造模后第7天,苏木精-伊红染色病理切片观察脑组织形态学,并检测血液黏度和炎症因子白细胞介素6、白细胞介素1β、肿瘤坏死因子α水平。结果与结论:①造模后第1天,模型组、改良模型组大鼠mNSS评分均高于假手术组(P<0.0001),改良模型组评分低于模型组(P<0.0001);②造模后第1,7天,模型组、改良模型组T2值均高于假手术组(P<0.05),改良模型组T2值低于模型组(P<0.05);③造模后第7天,模型组和改良模型组的血液黏度值、白细胞介素6、白细胞介素1β、肿瘤坏死因子α水平均高于假手术组(P<0.05),改良模型组白细胞介素6水平低于模型组(P<0.05);④基于Feeney自由落体法的创伤性脑损伤大鼠改良模型较好地控制了创伤性脑损伤大鼠造模中开颅骨的损伤因素。BACKGROUND:There is less report about mitigating sustained bone grinding injuries during craniotomy based on a model of traumatic brain injury established using the modified Feeney’s free-fall method.OBJECTIVE:To modify a modified traumatic brain injury model by altering the opening of the skull window.METHODS:Thirty-six Sprague-Dawley rats were equally randomized into sham group,model group and modified model group.The modified procedure of opening the bone window was used in the modified model group.Six to eight small holes of 0.3-0.5 mm in diameter were punched at the edge of the impact area and the drill was immediately withdrawn without touching the cortex.In the modified model group,the skull window was opened by using the modified method,while the skull window in the model group was opened using the conventional method.The modified model group and model group were established using the Feeney’s free-fall method.In the sham group,only the skull window was opened without impact.The modified neurological severity scoring was performed at 1 day after modeling.T2 weighted imaging was performed and T2 values were measured at 1 and 7 days after modeling.Hematoxylin-eosin staining of the brain section was made for histopathological observation at 7 days after modeling.The level of blood viscosity,interleukin-6,interleukin-1β,and tumor necrosis factor-αwere determined at 7 days after modeling.RESULTS AND CONCLUSION:Compared with the sham group,the modified neurological severity scores in the model group and modified model group were significantly increased at 1 day after modeling(P<0.0001).Meanwhile,the modified neurological severity scores in the modified model group were lower than those in the model group(P<0.0001).Compared with the sham group,the T2 values were significantly increased in the model group and modified model group at 1 and 7 days after modeling(P<0.05),while the T2 values in the modified model group were lower than those in the model group(P<0.05).Compared with the sham group,the level of bl

关 键 词：创伤性脑损伤 动物模型改良 行为学 炎症因子 大鼠 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R-332