CHST11基因对胃癌SGC-7901细胞生物学行为的影响  

Effects of CHST11 Gene on Biological Behavior of Gastric Cancer SGC-7901 Cells

作　　者：师文 韩炜[2] 孙焕焕 刘梦莹[1] 马富权 SHI Wen;HAN Wei;SUN Huanhuan;LIU Mengying;MA Fuquan(Department of Gastroenterology,the First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China;Department of Surgical Oncology,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)

机构地区：[1]西安交通大学第一附属医院消化内科,西安710061 [2]陕西省人民医院肿瘤外科,西安710068

出　　处：《中国细胞生物学学报》2023年第7期1020-1028,共9页Chinese Journal of Cell Biology

基　　金：陕西省自然科学基础研究计划(批准号:2023-JC-QN-0947、2021JQ-917)资助的课题。

摘　　要：该文探究了CHST11基因对胃癌SGC-7901细胞生物学行为的影响。应用q RT-PCR技术检测CHST11 m RNA在胃癌SGC-7901细胞中的表达情况。细胞转染干扰CHST11的sh-CHST11质粒和对照NC质粒,应用q RT-PCR及Western blot法分别检测CHST11基因稳定沉默后胃癌细胞CHST11 mRNA和蛋白的表达情况,应用转染后的细胞进行后续研究;采用MTT方法及平板克隆实验,分别观察CHST11基因稳定沉默对胃癌细胞增殖及克隆形成的影响;应用流式细胞技术检测转染后胃癌细胞的凋亡情况;通过划痕、Transwell实验,观察CHST11基因稳定沉默对胃癌细胞迁移、侵袭能力的影响。结果表明,CHST11基因在胃癌细胞中的表达上调;稳定沉默后的胃癌细胞SGC-7901-sh-CHST11的增殖、迁移及侵袭能力均显著低于未经干预的SGC-7901,SGC-7901-sh-CHST11细胞凋亡数明显增多;且SGC-7901-NC细胞的增殖、迁移、侵袭及凋亡情况与未经干预的SGC-7901相比均无显著差异。该研究结果表明,CHST11可促进胃癌细胞的增殖、迁移及侵袭,抑制凋亡,对胃癌的发生发展有促进作用。The effects of CHST11 gene on the biological behavior of gastric cancer SGC-7901 cells were in­vestigated.qRT-PCR and Western blot methods were used to detect CHST11 mRNA and protein expression in gastric cancer cells after the stable silencing of CHST11 gene.The cells were transfected with CHST11-interfering sh-CHST11 plasmid and control NC plasmid,and the CHST11 mRNA expression in gastric cancer cells after CHST11 gene knock­out was detected by qRT-PCR.By the MTT assay and the plate cloning experiment,the proliferation and cloning ca­pacities of the transfected gastric cancer cells separately were identified.Flow cytometry was used to identify the apop­tosis of gastric cancer cells following transfection.The capacity of stomach cancer cells to migrate and invade was determined using the scratch healing test and the Transwell assay,respectively.The results showed that the expression of CHST11 gene was up-regulated in gastric cancer cells.The capacity of SGC-7901-sh-CHST11 cells to proliferate,migrate and invade after stable transfection were significantly lower than that of SGC-7901 cells without intervention,and the number of apoptosis of SGC-7901-sh-CHST11 cells was significantly increased.The proliferation,migration,invasion and apoptosis of SGC-7901-NC cells were not significantly different from that of the untreated SGC-7901 cells.The results showed that CHST11 gene may enhance gastric cancer cell proliferation,migration,and invasion,as well as prevent apoptosis and promote the incidence and growth of gastric cancer cells.

关 键 词：胃癌 CHST11基因 增殖 凋亡 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]