A DnaA-dependent riboswitch for transcription attenuation of the his operon  

作　　者：Yuan Yao Hongwei Sun Wurihan Gegeheng Gezi Kirsten Skarstad Lifei Fan Morigen 

机构地区：[1]State Key Laboratory of Reproductive Regulation,Breeding of Grassland Livestock,School of Life Sciences,Inner Mongolia University,Hohhot,China [2]Department of Molecular Cell Biology and Department of Microbiology,Oslo University Hospital,Oslo,Norway

出　　处：《mLife》2023年第2期126-140,共15页微生物（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(Grant no.32260233 to Morigen);the Science and Technology Foundation of Inner Mongolia(Inner Mongolia Key Laboratory for Molecular Regulation of the Cell,Grant no.2021PT0002).

摘　　要：Transcription attenuation in response to the availability of a specific amino acid is believed to be controlled by alternative configurations of RNA secondary structures that lead to the arrest of translation or the release of the arrested ribosome from the leader mRNA molecule.In this study,we first report a possible example of the DnaA‐dependent riboswitch for transcription attenuation in Escherichia coli.We show that(i)DnaA regulates the transcription of the structural genes but not that of the leader hisL gene;(ii)DnaA might bind to rDnaA boxes present in the HisL‐SL RNA,and subsequently attenuate the transcription of the operon;(iii)the HisL‐SL RNA and rDnaA boxes are phylogenetically conserved and evolutionarily important;and(iv)the translating ribosome is required for deattenuation of the his operon,whereas tRNA^(His) strengthens attenuation.This mechanism seems to be phylogenetically conserved in Gram‐negative bacteria and evolutionarily important.

关 键 词：DnaA-dependent Escherichia coli his operon RIBOSWITCH transcription attenuation 

分 类 号：Q93[生物学—微生物学]