Genome-wide identification of endogenous retrovirus elements and their active transcription in mink genome  

作　　者：Zheng Li Qing Wang Na Lv Guojin Xu Xuemei Yang Baoli Zhu 

机构地区：[1]CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China [2]University of Chinese Academy of Sciences,Beijing,China [3]Jiangxi Science and Technology Normal University,Nanchang,China [4]Jinan Microecological Biomedicine Shandong Laboratory,Jinan,China [5]Beijing Pediatric Research Institute,Beijing,China [6]Department of Pathogenic Biology,School of Basic Medical Sciences,Southwest Medical University,Luzhou,China

出　　处：《mLife》2023年第2期201-208,共8页微生物（英文）

基　　金：supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29020203);the National Natural Science Foundation of China(32170068);the Research Project of Jinan Microecological Biomedicine Shandong Laboratory(JNL-2022013B).

摘　　要：Mammalian endogenous retroviruses(ERVs)are ancient retroviruses that have been integrated into genomes.ERVs were believed to be inactive until the discovery of ERV transcription in the mouse genome.However,the transcription level and function of ERV elements in mammalian genomes are not well understood.In this study,we performed the first genome‐wide scanning of ERV loci in the American mink(Neogale vison)genome(NeoERV)followed by transcriptomic analysis to detect actively transcribed NeoERV elements.A total of 365,791 NeoERV loci were identified,and161,205(44%)of these loci were found to be actively transcribed based on transcriptomic data from three types of tissues(amygdala,trachea and lung).More than one third of the actively transcribed NeoERV loci were tissue‐specific.Furthermore,some of the active loci were associated with host gene transcription,and the level of NeoERV transcription was positively correlated with that of host genes,specifically when active loci were located in overlapped gene regions.An in‐depth analysis of the envelope protein coding env gene showed that,in general,its transcription level was higher than that of NeoERVs,which is believed to be associated with host immunity.

关 键 词：endogenous retrovirus MINK TISSUE-SPECIFIC TRANSCRIPTOME 

分 类 号：Q943[生物学—植物学]