中华猕猴桃DLD2基因的生物信息学分析  

Bioinformatics Analysis of DLD2 Gene from Actinidia chinensis

作　　者：邓茹友 杨彦萍 张先昂 杨文俊 张汉尧[1] Deng Ruyou;Yang Yanping;Zhang Xian'ang;Yang Wenjun;Zhang Hanyao(Key Laboratory for Forest Genetic and Tree Improvement&Propagation in Universities of Yunnan Province,Southwest Forestry University,Kunming,650224;Key Laboratory of Biodiversity Conservation in Southwest China,State Forest Administration,Southwest Forestry University,Kunming,650224)

机构地区：[1]西南林业大学,云南省高校林木遗传改良与繁育重点实验室,昆明650224 [2]西南林业大学,西南地区生物多样性保育国家林业局重点实验室,昆明650224

出　　处：《分子植物育种》2023年第19期6308-6314,共7页Molecular Plant Breeding

基　　金：云南省自然科学基金项目(31760450)资助。

摘　　要：二氢硫辛酰胺脱氢酶(DLD2)是一种广泛存在于各种生物而且在能量代谢中起到重要作用的氧化还原酶,植物体内抗氧化酶在清除活性氧的过程中发挥重要作用,通过调控中华猕猴桃酶活性水平从而提高抗旱性。为了探究DLD2基因在中华猕猴桃这种不耐旱树种的功能,本研究使用同源物种分析法对中华猕猴桃转录组中的二氢硫辛酰胺脱氢酶(DLD2)基因序列进行分析,利用网上在线分析工具分析其编码蛋白质的结构并预测其功能。研究结果表明,该核苷酸序列的开放阅读框为1605bp,可编码534个氨基酸,丙氨酸所占比例为9.9%、甘氨酸所占比例为9.4%以及脯氨酸所占含量为3.9%;在亲疏水性分析中得出该基因编码的蛋白是一个亲水性蛋白;跨膜结构分析中也得出该蛋白全部位于细胞膜外部,不含有跨膜结构;蛋白质motif搜索中,也得出含有氧化还原酶位点,使得该基因中酶的活性大大提高;结构域中存在Pyr_redox结构域,它属于吡啶核苷酸二硫键氧化还原酶,初步推测对中华猕猴桃能起到氧化代谢作用。该研究结果可为后续的基因功能确证以及中华猕猴桃品种改良提供了依据。Dihydrolipoamide dehydrogenase(DLD2)is an oxidoreductase that is widely present in various organisms and plays an important role in energy metabolism.The antioxidant enzyme in plants plays an important role in the process of removing reactive oxygen species.Improve drought resistance by regulating the enzyme activity level of Actinidia chinensis.In order to explore the function of DLD2 gene in the drought-tolerant tree species of Actinidia chinensis,in this study,the dihydrothiamide dehydrogenase(DLD2)gene sequence in the transcriptome of Actinidia chinensis was analyzed using homologous species analysis.Use online analysis tools to analyze the structure of the protein and predict its function.The results showed that the open reading frame of the nucleotide sequence is 1605 bp,which can encode 534 amino acids,among them,the proportion of alanine,glycine and proline is 9.9%,9.4%and 3.9%,respectively.In the hydrophilic analysis,it is concluded that the protein encoded by this gene is a hydrophilic protein.In the transmembrane structure analysis,it is also found that the protein is all located outside the cell membrane and does not contain a transmembrane structure.In the protein motif search,it is also concluded that there are oxidoreductase sites,which greatly improves the enzyme activity in the gene.The Pyr_redox domain exists in the domain,which belongs to the pyridine nucleotide disulfide bond oxidoreductase,and it is preliminarily speculated that it can play an oxidative metabolism on Actinidia chinensis.The results provided a basis for the subsequent confirmation of gene function and the improvement of Actinidia chinensis varieties.

关 键 词：中华猕猴桃 DLD2 抗旱 生物信息学方法 

分 类 号：S663.4[农业科学—果树学]