Baicalin,silver titanate,Bletilla striata polysaccharide and carboxymethyl chitosan in a porous sponge dressing for burn wound healing  

作　　者：Yan-rong Gong Cheng Zhang Xing Xiang Zhi-bo Wang Yu-qing Wang Yong-hua Su Hui-qing Zhang 

机构地区：[1]School of Traditional Chinese Medicine,Naval Medical University,Shanghai 200433,China [2]Department of Dermatology,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [3]Department of Dermatology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China [4]Institute of Dermatology,Shanghai Academy of Traditional Chinese Medicine,Shanghai 201203,China

出　　处：《Journal of Integrative Medicine》2023年第5期487-495,共9页结合医学学报（英文版）

基　　金：The work was supported by grants from the Dark Blue 123 Project of First Affliated Hospital of Naval Medical University(Foundation No.2019SLZ015);Youth Cultivation Project of Military Medical Science(Foundation No.14QNP083);the Clinical Research Plan of SHDC(Foundation No.SHDC2020CR3097B).

摘　　要：Objective This study tests the efficacy of Bletilla striata polysaccharide(BSP),carboxymethyl chitosan(CMC),baicalin(BA)and silver titanate(ST)in a wound dressings to fight infection,promote healing and provide superior biocompatibility.Methods The antibacterial activity of BA and ST was evaluated in vitro using the inhibition zone method.BA/ST/BSP/CMC porous sponge dressings were prepared and characterized.The biocompatibility of BA/ST/BSP/CMC was assessed using the cell counting kit-8 assay.The therapeutic effect of BA/ST/BSP/CMC was further investigated using the dorsal skin burn model in Sprague-Dawley rats.Results The wound dressing had good antibacterial activity against Escherichia coli and Staphylococcus aureus through BA and ST,while the combination of BSP and CMC played an important role in promoting wound healing.The BA/ST/BSP/CMC porous sponge dressings were prepared using a freeze-drying method with the concentrations of BA and ST at 20 and 0.83 mg/mL,respectively,and the optimal ratio of 5%BSP to 4%CMC was 1:3.The average porosity,water absorption and air permeability of BA/ST/BSP/CMC porous sponge dressings were measured to be 90.43%,746.1%and 66.60%,respectively.After treatment for 3 and 7 days,the healing rates of the BA/ST/BSP/CMC group and BA/BSP/CMC group were significantly higher than those of the normal saline(NS)group and silver sulfadiazine(SSD)group(P<0.05).Interleukin-1βexpression in the BA/ST/BSP/CMC group at 1 and 3 days was significantly lower than that in the other three groups(P<0.05).After being treated for 3 days,vascular endothelial growth factor expression in the BA/BSP/CMC group and BA/ST/BSP/CMC group was significantly higher than that in the NS group and SSD group(P<0.05).Inspection of histological sections showed that the BA/ST/BSP/CMC group and BA/BSP/CMC group began to develop scabbing and peeling of damaged skin after 3 days of treatment,indicating accelerated healing relative to the NS group and SSD group.Conclusion The optimized concentration of BA/ST/BSP/CMC dressing

关 键 词：Wound dressing BAICALIN Bletilla striata polysaccharide Silver titanate CARBOXYMETHYLCHITOSAN 

分 类 号：R285.5[医药卫生—中药学]