Microneedle array facilitates hepatic sinusoid construction in a large-scale liver-acinus-chip microsystem  

作　　者：Shibo Li Chengpan Li Muhammad Imran Khan Jing Liu Zhengdi Shi Dayong Gao Bensheng Qiu Weiping Ding 

机构地区：[1]Department of Electronic Engineering and Information Science,University of Science and Technology of China,Hefei,Anhui 230027,China [2]Department of Oncology,the First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230001,China [3]Center for Biomedical Imaging,University of Science and Technology of China,Hefei,Anhui 230027,China [4]School of Biology,Food and Environment,Hefei University,Hefei,Anhui 230601,China [5]Department of Mechanical Engineering,University of Washington,Seattle,WA 98195,USA

出　　处：《Microsystems & Nanoengineering》2023年第3期333-346,共14页微系统与纳米工程（英文）

基　　金：We acknowledge the funding support from the National Natural Science Foundation of China(82072018,81571768);the Anhui Provincial Natural Science Foundation(2208085QH256);the Strategic Priority Research Program(C)of the CAS(XDC07040200);the China Postdoctoral Science Foundation(2022M713055);the National Key Research and Development Program of China(2018AAA0100300);the Fundamental Research Funds for Central Universities(WK2480000006,WK9100000001,WK9110000125)。

摘　　要：Hepatic sinusoids play a key role in maintaining high activities of liver cells in the hepatic acinus.However,the construction of hepatic sinusoids has always been a challenge for liver chips,especially for large-scale liver microsystems.Herein,we report an approach for the construction of hepatic sinusoids.In this approach,hepatic sinusoids are formed by demolding a self-developed microneedle array from a photocurable cell-loaded matrix in a large-scale liver-acinus-chip microsystem with a designed dual blood supply.Primary sinusoids formed by demolded microneedles and spontaneously self-organized secondary sinusoids can be clearly observed.Benefiting from significantly enhanced interstitial flows by formed hepatic sinusoids,cell viability is witnessed to be considerably high,liver microstructure formation occurs,and hepatocyte metabolism is enhanced.In addition,this study preliminarily demonstrates the effects of the resulting oxygen and glucose gradients on hepatocyte functions and the application of the chip in drug testing.This work paves the way for the biofabrication of fully functionalized large-scale liver bioreactors.

关 键 词：NEEDLE HEPATIC METABOLISM 

分 类 号：TB383[一般工业技术—材料科学与工程] R575[医药卫生—消化系统]