A comparative study on environmental surveillance of enterovirus:Using a two-phase separation method and a filtration method with a mixed cellulose ester(MCE)membrane  

作　　者：Ling Fang Meizhong Chen Shuangli Zhu Wei Zhang Dongmei Yan Xiaolei Li Shufen Huang Caixia Li Xue Guo Hanri Zeng Bixia Ke Hui Li Wenbo Xu Changwen Ke Xiaoling Deng Yong Zhang Huanying Zheng 

机构地区：[1]Guangdong Provincial Center for Disease Control and Prevention,Guangdong Workstation for Emerging Infectious Disease Control and Prevention,Guangzhou 511430,China [2]WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China

出　　处：《Biosafety and Health》2023年第3期174-180,共7页生物安全与健康（英文）

基　　金：supported by the National Key Research and Development Program of China(Project No.2021YFC2302003);the Natural Science Foundation of Beijing(Project No.L192014).

摘　　要：This study aimed to compare the sensitivity of two-phase separation and the filtration method using a mixed cellulose ester(MCE)membrane to detect enteroviruses in sewage samples.From December 2015 to July 2016,four domestic sewage samples(1 L/sample)were collected monthly from the Guangzhou Liede Sewage Treatment Plant,and each sewage sample was divided into two aliquots(500 mL).The sewage sample was concentrated using the two-phase separation and the filtration method using an MCE membrane,and the treated solutions were inoculated into cells for enterovirus isolation.Polymerase chain reaction amplification,VP1 sequencing,and enterovirus molecular typing were performed on the positive isolates.The detection rates of poliovirus(PV)and non-polio enterovirus(NPEV)obtained using the filtration method using an MCE membrane were higher than those using the two-phase separation method.McNemar's test showed that the detection rates of PV,NPEV,type 1 Sabin-like(SL1),type 2 Sabin-like(SL2),and type 3 Sabin-like(SL3)strain were not statistically significant(P>0.05).In Guangdong Province,China,the detection rates for PV and NPEV were 53.13%and 62.50%(20/32),respectively.Twenty-seven PVs were isolated,three highly variable strains of the type 1 vaccine,with seven nucleotide substitutions in the VP1 region,compared with the type 1 Sabin strains.Eighty-seven strains of NPEV were isolated and nine serotypes were identified,among which coxsackievirus B3(CVB3),echovirus 6(E6),and echovirus 11(E11)were the dominant strains.The filtration method using an MCE membrane is more sensitive than two-phase separation and can be used as a robust,sensitive,and cost-effective method to isolate enteroviruses from sewage.

关 键 词：Mixed cellulose ester Two-phase separation Environmental surveillance POLIOVIRUS ENTEROVIRUS 

分 类 号：R373[医药卫生—病原生物学]